A subc lipoma peri pre pre peri pre

A subc lipoma peri pre pre peri pre D peri lipoma subc E subc C lipoma subc B PTEN Supplementary Figure 1: Gene expression in different adipose tissue depots of marker genes for A) brown adipocytes: PR Domain 16 (PRDM 16), B) endothelial cells: cadherin 5 (CDH 5), C) macrophages: cluster of differentiation (CD)163 and D) fibroblasts: Cartilage Oligomeric Matrix Protein (COMP). Data are normalised to hypoxanthine phosphoribosyltransferase (HPRT ) and TATA box binding protein (TBP) expression and presented as mean±SEM. , n = 3 independent experiments; subc: subcutaneous, peri: perirenal, pre: prerenal E) Schematic depiction of heterozygous PTEN deletion: size of deletion: 152. 100 bp, position: chr. 10, 89. 629. 799 – 89. 781. 899, green bar: PTEN exon 1, purple arrowed bar: deletion, black arrowed bar: PTEN gene.

A cells/cm² B C 48 72 Time (h) 96 PPARγ # # 0 # 8 12 0 4 8 12 Day of differentiation # 0 F 4 8 12 0 4 8 12 Day of differentiation # FASN ADIPONECTIN E 4 D Lip. PD 1 SGBS a. P 2 24 # # 0 4 8 12 0 4 Day of differentiation 8 12 0 4 8 12 Day of differentiation Supplementary Figure 2: Characteristics of Lip. PD 1 cells A) Doubling time was approximately 25 h, number of cells were counted after 24, 48, 72 and 96 h in 20 -cates and presented as mean±SEM, n = 2. B) Long-term culture of Lip. PD 1 cells until growth is attenuated. n = 1; Expression of white adipocyte marker genes C) peroxisome proliferator-activated receptor γ (PPARy), D) adipocyte binding protein 2 (a. P 2), E) adiponectin and F) fatty acid synthase. Data are presented as mean±SEM, n = 3 independent experiments; #p<0. 05 determined by two-way ANOVA comparing Lip. PD 1 to SGBS preadipocytes

A B Lip. PD 1 SGBS p. AKT(Thr 308)/AKT # p. AKT 0 0. 01 0. 1 1 10 100 IGF-I (n. M) C p. AKT(Ser 473)/AKT # p. AKT # 0 0. 01 0. 1 1 10 100 IGF-I (n. M) Supplementary Figure 3: Basal and IGF-I stimulated AKT phosphorylation in SGBS and Lip. PD 1 preadipocytes A) Thr 308, B) Ser 473, presented as mean±SEM, n = 4 independent experiments; #p<0. 05 determined by two-way ANOVA comparing Lip. PD 1 to SGBS preadipocytes.

A Lip. PD 1 p. AKT(Thr 308)/AKT SGBS p. AKT 0 0. 01 0. 1 1 10 100 Insulin (n. M) p. AKT(Ser 473)/AKT B p. AKT 0 0. 01 0. 1 1 10 100 Insulin (n. M) Supplementary Figure 4: Basal and insulin-stimulated AKT phosphorylation in SGBS and Lip. PD 1 adipocytes A) Thr 308, B) Ser 473, presented as mean±SEM, n = 4 independent experiments.