A saturating mutagenesis CRISPRCas 9mediated functional genomic screen

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A saturating mutagenesis CRISPR-Cas 9–mediated functional genomic screen identifies cis- and trans-regulatory elements of

A saturating mutagenesis CRISPR-Cas 9–mediated functional genomic screen identifies cis- and trans-regulatory elements of Oct 4 in murine ESCs Matthew C. Canver, Pratibha Tripathi, Michael J. Bullen, Moshe Olshansky, Yogesh Kumar, Lee H. Wong, Stephen J. Turner, Samuel Lessard, Luca Pinello, Stuart H. Orkin, Partha Pratim Das Journal of Biological Chemistry Volume 295 Issue 47 Pages 15797 -15809 (November 2020) DOI: 10. 1074/jbc. RA 120. 013772 Copyright © 2020 Canver et al. Terms and Conditions

Figure 1 Journal of Biological Chemistry 2020 29515797 -15809 DOI: (10. 1074/jbc. RA 120.

Figure 1 Journal of Biological Chemistry 2020 29515797 -15809 DOI: (10. 1074/jbc. RA 120. 013772) Copyright © 2020 Canver et al. Terms and Conditions

Figure 2 Journal of Biological Chemistry 2020 29515797 -15809 DOI: (10. 1074/jbc. RA 120.

Figure 2 Journal of Biological Chemistry 2020 29515797 -15809 DOI: (10. 1074/jbc. RA 120. 013772) Copyright © 2020 Canver et al. Terms and Conditions

Figure 3 Journal of Biological Chemistry 2020 29515797 -15809 DOI: (10. 1074/jbc. RA 120.

Figure 3 Journal of Biological Chemistry 2020 29515797 -15809 DOI: (10. 1074/jbc. RA 120. 013772) Copyright © 2020 Canver et al. Terms and Conditions

Figure 4 Journal of Biological Chemistry 2020 29515797 -15809 DOI: (10. 1074/jbc. RA 120.

Figure 4 Journal of Biological Chemistry 2020 29515797 -15809 DOI: (10. 1074/jbc. RA 120. 013772) Copyright © 2020 Canver et al. Terms and Conditions

Figure 5 Journal of Biological Chemistry 2020 29515797 -15809 DOI: (10. 1074/jbc. RA 120.

Figure 5 Journal of Biological Chemistry 2020 29515797 -15809 DOI: (10. 1074/jbc. RA 120. 013772) Copyright © 2020 Canver et al. Terms and Conditions

Figure 6 Journal of Biological Chemistry 2020 29515797 -15809 DOI: (10. 1074/jbc. RA 120.

Figure 6 Journal of Biological Chemistry 2020 29515797 -15809 DOI: (10. 1074/jbc. RA 120. 013772) Copyright © 2020 Canver et al. Terms and Conditions

Introduction Sitespecific mutagenesis The cause of mutagenesis TheIntroduction Sitespecific mutagenesis The cause of mutagenesis The
Sitespecific mutagenesis DNA PCR Sitespecific mutagenesis Horton 1997Sitespecific mutagenesis DNA PCR Sitespecific mutagenesis Horton 1997
Directed Mutagenesis and Protein Engineering Directed Mutagenesis MutationrandomDirected Mutagenesis and Protein Engineering Directed Mutagenesis Mutationrandom
SITEDIRECTED MUTAGENESIS SITEDIRECTED MUTAGENESIS Make a specific desiredSITEDIRECTED MUTAGENESIS SITEDIRECTED MUTAGENESIS Make a specific desired
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