A saturating mutagenesis CRISPRCas 9mediated functional genomic screen Slides: 7 Download presentation A saturating mutagenesis CRISPR-Cas 9–mediated functional genomic screen identifies cis- and trans-regulatory elements of Oct 4 in murine ESCs Matthew C. Canver, Pratibha Tripathi, Michael J. Bullen, Moshe Olshansky, Yogesh Kumar, Lee H. Wong, Stephen J. Turner, Samuel Lessard, Luca Pinello, Stuart H. Orkin, Partha Pratim Das Journal of Biological Chemistry Volume 295 Issue 47 Pages 15797 -15809 (November 2020) DOI: 10. 1074/jbc. RA 120. 013772 Copyright © 2020 Canver et al. Terms and Conditions Figure 1 Journal of Biological Chemistry 2020 29515797 -15809 DOI: (10. 1074/jbc. RA 120. 013772) Copyright © 2020 Canver et al. Terms and Conditions Figure 2 Journal of Biological Chemistry 2020 29515797 -15809 DOI: (10. 1074/jbc. RA 120. 013772) Copyright © 2020 Canver et al. Terms and Conditions Figure 3 Journal of Biological Chemistry 2020 29515797 -15809 DOI: (10. 1074/jbc. RA 120. 013772) Copyright © 2020 Canver et al. Terms and Conditions Figure 4 Journal of Biological Chemistry 2020 29515797 -15809 DOI: (10. 1074/jbc. RA 120. 013772) Copyright © 2020 Canver et al. Terms and Conditions Figure 5 Journal of Biological Chemistry 2020 29515797 -15809 DOI: (10. 1074/jbc. RA 120. 013772) Copyright © 2020 Canver et al. Terms and Conditions Figure 6 Journal of Biological Chemistry 2020 29515797 -15809 DOI: (10. 1074/jbc. RA 120. 013772) Copyright © 2020 Canver et al. Terms and Conditions CRISPRCas 9 and Active Genetics Valentino Gantz CRISPRCasIntroduction Sitespecific mutagenesis The cause of mutagenesis TheSitespecific mutagenesis DNA PCR Sitespecific mutagenesis Horton 1997Directed Mutagenesis and Protein Engineering Directed Mutagenesis MutationrandomSitedirected mutagenesis IRSHAD PHD Sitedirected mutagenesis is aDirected Mutagenesis and Protein Engineering Directed Mutagenesis MutationrandomSITEDIRECTED MUTAGENESIS SITEDIRECTED MUTAGENESIS Make a specific desired