A role for activated endothelial cells in red

  • Slides: 9
Download presentation
A role for activated endothelial cells in red blood cell clearance: implications for vasopathology

A role for activated endothelial cells in red blood cell clearance: implications for vasopathology by Marcel H. A. M. Fens, Richard van Wijk, Grietje Andringa, Karlijn L. van Rooijen, Hilde M. Dijstelbloem, Jan T. Rasmussen, Karen M. K. de Vooght, Raymond M. Schiffelers, Carlo A. J. M. Gaillard, and Wouter W. van Solinge haematol Volume 97(4): 500 -508 April 5, 2012 © 2012 by Ferrata Storti Foundation

Overview of flow cytometry parameters. Marcel H. A. M. Fens et al. Haematologica 2012;

Overview of flow cytometry parameters. Marcel H. A. M. Fens et al. Haematologica 2012; 97: 500 -508 © 2012 by Ferrata Storti Foundation

Phosphatidylserine (PS) exposure of RBC induced by the calcium ionophore A 23187 and oxidative

Phosphatidylserine (PS) exposure of RBC induced by the calcium ionophore A 23187 and oxidative stress (t. BHP). Marcel H. A. M. Fens et al. Haematologica 2012; 97: 500 -508 © 2012 by Ferrata Storti Foundation

Mechanical alterations of RBC induced by calcium ionophore and oxidative stress (t. BHP). Marcel

Mechanical alterations of RBC induced by calcium ionophore and oxidative stress (t. BHP). Marcel H. A. M. Fens et al. Haematologica 2012; 97: 500 -508 © 2012 by Ferrata Storti Foundation

Overview of the degree of hemolysis. Marcel H. A. M. Fens et al. Haematologica

Overview of the degree of hemolysis. Marcel H. A. M. Fens et al. Haematologica 2012; 97: 500 -508 © 2012 by Ferrata Storti Foundation

Erythrophagocytosis by endothelial cells (HUVEC) of PS-exposing RBC induced by the calcium ionophore A

Erythrophagocytosis by endothelial cells (HUVEC) of PS-exposing RBC induced by the calcium ionophore A 23187 and oxidative stress (t. BHP). Marcel H. A. M. Fens et al. Haematologica 2012; 97: 500 -508 © 2012 by Ferrata Storti Foundation

Imaging of erythrophagocytosis by HUVEC of oxidative stress (3 m. M t. BHP)-stimulated RBC.

Imaging of erythrophagocytosis by HUVEC of oxidative stress (3 m. M t. BHP)-stimulated RBC. (A) Overview of erythrophagocytosis of lactadherin-incubated t. BHP-stimulated RBC in time. Marcel H. A. M. Fens et al. Haematologica 2012; 97: 500 -508 © 2012 by Ferrata Storti Foundation

Perfusion of oxidative stress (3 m. M t. BHP)-stimulated RBC over HUVEC. Images taken

Perfusion of oxidative stress (3 m. M t. BHP)-stimulated RBC over HUVEC. Images taken during and after perfusion (shear rate 100 s− 1) of 3 m. M t. BHP-stimulated RBC over HUVEC, (A) in the absence of lactadherin no association was seen whereas (B) in the presence o. . . Marcel H. A. M. Fens et al. Haematologica 2012; 97: 500 -508 © 2012 by Ferrata Storti Foundation

Cytotoxic effect of erythrophagocytosis on HUVEC. Red blood cells (RBC) treated with 3 m.

Cytotoxic effect of erythrophagocytosis on HUVEC. Red blood cells (RBC) treated with 3 m. M t. BHP were pre-incubated with lactadherin and incubated with HUVEC for 4 h. Marcel H. A. M. Fens et al. Haematologica 2012; 97: 500 -508 © 2012 by Ferrata Storti Foundation

A role for activated endothelial cells in redA role for activated endothelial cells in red
Activated carbon also called activated charcoal activated coalActivated carbon also called activated charcoal activated coal
SYSTEMIC SCLEROSIS Pathogenesis Vasculopathy Activated endothelial cells releaseSYSTEMIC SCLEROSIS Pathogenesis Vasculopathy Activated endothelial cells release
Glutamine reduces ICAM1 expression in endothelial cells activatedGlutamine reduces ICAM1 expression in endothelial cells activated
Protease Activated Receptor2 Mediates Activated Protein CInduced CutaneousProtease Activated Receptor2 Mediates Activated Protein CInduced Cutaneous
Red acadmica y Red comercial Red Acadmica RedRed acadmica y Red comercial Red Acadmica Red
Proteomic Analysis of Vascular Endothelial Growth Factorinduced EndothelialProteomic Analysis of Vascular Endothelial Growth Factorinduced Endothelial
RED BLOOD CELLS Red blood cells are oneRED BLOOD CELLS Red blood cells are one