A ORF 57 CTD L 175 D 184

A ORF 57 -CTD L 175 D 184 S 187 I 194 R 189 R 197 ARM C 202 V 205 F 206 P 208 A 209 1 2 3 ARM 1 L 175 R 189 R 197 A 209 S 187 I 194 P 208 D 184 F 206 V 205 C 202 2 3 Q 280 4 5 6 4 Q 280 5 6 E 366 V 367 7 D 243 D 246 T 249 R 254 R 259 1 ARM S 272 R 271 2 1 ARM R 259 R 254 2 R 271 S 272 R 372 8 7 8 V 367 R 372 E 366 ICP 27 -CTD T 249 D 246 D 243 R 408 Y 406 T 402 L 399 E 398 R 395 9 L 419 A 420 I 422 K 423 3 4 5 6 7 9 R 395 E 398 L 399 T 402 Y 406 R 408 R 435 8 hydrogen bonds salt bridges N 417 D 411 10 10 D 411 N 417 H 461 8 R 435 K 423 H 461 I 422 R 480 D 477 E 474 9 10 B ARM 1 2 A 275 R 271 3 3 R 271 A 275 V 310 Q 299 R 309 A R P 279 297 308 ICP 27 -CTD 1 ARM 2 3 P 279 Q 299 R 308 A 297 R 309 V 310 3 R 327 R 325 5 4 4 R 345 C 342 S 334 R 340 4 4 S 334 R 340 T 335 C 342 R 345 5 R 327 R 325 Y 349 6 A 352 M 353 7 6 Y 349 E 358 S 355 5 T 392 D 388 R 395 6 5 S 355 E 358 6 D 388 R 395 T 392 7 M 353 A 352 11 R 437 Y 452 E 474 D 477 R 480 A 420 L 419 ORF 57 -CTD R 437 Y 452 11 hydrogen bonds salt bridges 8 9 10 7 8 M 469 P 465 9 9 P 465 M 469 G 466 E 431 11 11 E 431 F 506 Y 505 K 504 G 503 T 491 A 502 R 486 V 501 10 Y 507 C 508 D 509 S 510 F 512 10 R 486 K 504 Y 507 T 491 A 502 D 509 V 501 S 510 F 512 Supplemental Figure S 5. Comparison of polar intermolecular interactions within ORF 57 and ICP 27 dimer. The diagrams illustrates the polar intermolecular interactions between “arm” (green box) and globular (yellow box) domains (a) and between two globular domains (b) in the ORF 57 dimer and ICP 27 dimer (PDB ID: 4 yxp). The numbered yellow boxes represent individual α-helixes. The dash lines of ORF 57 and ICP 27 show hydrogen bonds (blue lines) or salt bridges (red lines) between interacting residues. Interface interaction analyses of ORF 57 and ICP 27 were done by using PDBe-PISA (http: //www. ebi. ac. uk/msd-srv/prot_int/cgi-bin/piserver) and the interface interaction residues of ORF 57 are also listed in Supplemental Table S 4.