A novel validated enzymelinked immunosorbent assay to quantify

A novel validated enzyme-linked immunosorbent assay to quantify soluble hemojuvelin in mouse serum by Wenjie Chen, Chia Chi Sun, Shanzhuo Chen, Delphine Meynard, Jodie L. Babitt, and Herbert Y. Lin haematol Volume 98(2): 296 -304 January 31, 2013 © 2013 by Ferrata Storti Foundation

Specificity of monoclonal antibody (m. Ab) anti-HJV 18 H 8 and polyclonal antibody (p. Ab) anti-HJV and validation of the ELISA using cell-conditioned media containing s. HJV. Wenjie Chen et al. Haematologica 2013; 98: 296 -304 © 2013 by Ferrata Storti Foundation

Summary of the s. HJV ELISA characteristics using TBS-Casein as the diluent buffer. Wenjie Chen et al. Haematologica 2013; 98: 296 -304 © 2013 by Ferrata Storti Foundation

Quantitation of s. HJV in mouse serum by ELISA. (A) The standard curve for the s. HJV ELISA was generated using 2 -fold dilutions from 16 to 0. 5 ng/m. L of m. HJV. His protein as a calibrator diluted in Hjv-null mouse serum and plotted in a linear curve with measured. . . Wenjie Chen et al. Haematologica 2013; 98: 296 -304 © 2013 by Ferrata Storti Foundation

Summary of s. HJV ELISA characteristics using Hjv-null mouse serum as diluent buffer. Wenjie Chen et al. Haematologica 2013; 98: 296 -304 © 2013 by Ferrata Storti Foundation

Quantitation of serum s. HJV in mice after acute low iron diet treatment. Wenjie Chen et al. Haematologica 2013; 98: 296 -304 © 2013 by Ferrata Storti Foundation

Quantitation of serum s. HJV in mice after acute high iron diet treatment. Wenjie Chen et al. Haematologica 2013; 98: 296 -304 © 2013 by Ferrata Storti Foundation