A novel nanoimmunoassay method for quantification of proteins

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A novel nano-immunoassay method for quantification of proteins from CD 138 -purified myeloma cells:

A novel nano-immunoassay method for quantification of proteins from CD 138 -purified myeloma cells: biological and clinical utility by Irena Misiewicz-Krzeminska, Luis Antonio Corchete, Elizabeta A. Rojas, Joaquín Martínez-López, Ramón García-Sanz, Albert Oriol, Joan Bladé, Juan-José Lahuerta, Jesús San Miguel, María-Victoria Mateos, and Norma C. Gutiérrez haematol Volume 103(5): 880 -889 April 30, 2018 © 2018 by Ferrata Storti Foundation

Scheme of the study. Irena Misiewicz-Krzeminska et al. Haematologica 2018; 103: 880 -889 ©

Scheme of the study. Irena Misiewicz-Krzeminska et al. Haematologica 2018; 103: 880 -889 © 2018 by Ferrata Storti Foundation

Summary of proteins and antibodies used in the study. Irena Misiewicz-Krzeminska et al. Haematologica

Summary of proteins and antibodies used in the study. Irena Misiewicz-Krzeminska et al. Haematologica 2018; 103: 880 -889 © 2018 by Ferrata Storti Foundation

Optimization of protein extraction from RLT Plus samples. Irena Misiewicz-Krzeminska et al. Haematologica 2018;

Optimization of protein extraction from RLT Plus samples. Irena Misiewicz-Krzeminska et al. Haematologica 2018; 103: 880 -889 © 2018 by Ferrata Storti Foundation

Optimization of protein expression quantification. Irena Misiewicz-Krzeminska et al. Haematologica 2018; 103: 880 -889

Optimization of protein expression quantification. Irena Misiewicz-Krzeminska et al. Haematologica 2018; 103: 880 -889 © 2018 by Ferrata Storti Foundation

Two levels of analysis of each gene RNA and protein. m. RNA expression of

Two levels of analysis of each gene RNA and protein. m. RNA expression of each gene was assessed by q. RT-PCR and normalized relative to GAPDH and expressed as 2−ΔCt (A). Irena Misiewicz-Krzeminska et al. Haematologica 2018; 103: 880 -889 © 2018 by Ferrata Storti Foundation

Univariate analysis of progression-free survival. Irena Misiewicz-Krzeminska et al. Haematologica 2018; 103: 880 -889

Univariate analysis of progression-free survival. Irena Misiewicz-Krzeminska et al. Haematologica 2018; 103: 880 -889 © 2018 by Ferrata Storti Foundation

Progression-free survival according to levels of MRNA and protein expression. Irena Misiewicz-Krzeminska et al.

Progression-free survival according to levels of MRNA and protein expression. Irena Misiewicz-Krzeminska et al. Haematologica 2018; 103: 880 -889 © 2018 by Ferrata Storti Foundation

Progression-free survival in patients with low and high Cereblon, Aiolos and Ikaros protein levels,

Progression-free survival in patients with low and high Cereblon, Aiolos and Ikaros protein levels, depending on the treatment scheme (only patients treated according to GEM 2010 trial received lenalidomide). Irena Misiewicz-Krzeminska et al. Haematologica 2018; 103: 880 -889 © 2018 by Ferrata Storti Foundation