A New and Better Forensic Test for Human

A New and Better Forensic Test for Human Blood Karl Reich, Ph. D. Independent Forensics Lombard IL SPERM HY LITER

BLOOD extract in provided extraction buffer adsorb stain onto swab 15 min add to RSID-Blood mix extract aliquot with running buffer (100 ml total) RSIDTM Running Buffer SPERM HY LITER

BLOOD extract in provided extraction buffer adsorb stain onto swab 15 min add to RSID-Blood mix extract aliquot with running buffer (100 ml total) RSIDTM Running Buffer SPERM HY LITER

BLOOD Sensitivity Testing 50 ml of human blood extracted in 1 ml BEB Extract volume 0 ml Equiv. vol human blood 0 ml 1 ml@1: 5 10 nl 1 ml 5 ml 20 ml 100 ml 50 nl 250 nl 1 ml 5 ml SPERM HY LITER

BLOOD No High Dose Hook Effect Total Extract Volume (ml) 0 1, 000 500 200 Equiv. volume human blood (ml) 0 5 10 25 SPERM HY LITER

BLOOD Testing RSID-BLOODTM for Species Specificity 20 ml of Extract 1 ml equivalent neg – negative control pos – positive control F – ferret C – bonobo chimpanzee G – gorilla neg pos F C G SPERM HY LITER

BLOOD Species Specificity: Direct Comparison RSID-BLOODTM neg 1 ml pos 20 ml 1 ml ferret 20 ml gorilla 1 ml orangutan Seratec Hemedirect neg H F G 20 ml O Hexagon OBTI SPERM HY LITER

BLOOD SPERM HY LITER

BLOOD Specificity Testing Animal Species Tested: ferret, orangutan, gorilla, lowland gorilla, baboon, spider monkey, skunk, bonobo chimpanzee, goat, sheep, pig, horse, cat, mouse, dog, bull, Box turtle, opossum, American Elk, mule deer, alpaca, Siberian tiger, Nubian goat, Body Fluids Tested: sweat, semen, urine, saliva, vaginal secretions, double mixtures, triple mixtures NO CROSS REACTIONS IDENTIFIED - INCLUDING PRIMATES – CONFIRMATORY FOR HUMAN BLOOD SPERM HY LITER

Questions Comments Samples Karl Reich Tel 708. 234. 1200 Fax 708. 978. 5115 karl@IFI-test. com www. IFI-test. com SPERM HY LITER Gripes