A GCL NBL ED 18 5 PND 3
A GCL NBL ED 18. 5 PND 3 PND 6 B GCL INL ONL PND 60 Figure 1
A GCL INL ONL GS CDH 11 Merge 40 x oil INL Cralbp CDH 11 Merge 100 x oil B INL CDH 11 160 k. Da Merge 40 x oil GCL C INL ONL Chx-10 40 x oil Chx-10 100 x oil CDH 11 40 x oil CDH 11 INL D INL HPC-1 Figure 2 CDH 11 ONL
ED 18. 5 PND 3 PND 6 PND 15 PND 60 A GCL INL ONL B GCL INL ONL Figure 3
TAg A B C Figure 4 cadherin-11
Cdh 11+/+; TAg+/- A Cdh 11+/-; TAg+/- Cdh 11 -/-; TAg+/- Average Retinal Area and TAg positive cells per Eye at PND 8 B 1 K-W: 140 E 5 2 K-W: 1 120 E 5 n=5 100 E 5 9000 8000 7000 6000 5000 4000 3000 2000 1000 80 E 5 2 60 E 5 40 E 5 20 E 5 Cdh 11+/+; TAg+/- Cdh 11+/-; TAg+/- Cdh 11 -/-; TAg+/- Ratio of Total TAg-positive Cells to Total Retinal Area % TAg +ve cells / retina (cells/pixels) C p=0. 83 p=0. 01 0. 10 0. 09 0. 08 0. 07 0. 06 0. 05 0. 04 0. 03 0. 02 0. 01 0. 00 K-W: p=0. 01 Cdh 11+/+; TAg+/- Figure 5 Cdh 11+/-; TAg+/- Cdh 11 -/-; TAg+/- TAg +ve [cells] Retinal Area [pixels] 160 E 5
Cdh 11+/+; TAg+/- A B Cdh 11+/-; TAg+/- Cdh 11 -/-; TAg+/- Percent Tumor Area per Retinal Area at PND 28 % Total Tumor Area per Retina [pixels] 9 8 K-W: p=0. 016 n=5 7 6 5 4 3 2 1 0 Cdh 11+/+; TAg+/- Figure 6 Cdh 11+/-; TAg+/- Cdh 11 -/-; TAg+/-
A Cdh 11+/+; TAg+/- Cdh 11+/-; TAg+/- Cdh 11 -/-; TAg+/- Average Retinal and Tumor Area per Eye at PND 84 1. 40 E+07 Retinal and Tumor Area [pixels] 1. 20 E+07 1 K-W 2 K-W p=0. 01 p=0. 26 1. 00 E+07 8. 00 E+06 6. 00 E+06 4. 00 E+06 2. 00 E+06 0. 00 E+00 Cdh 11+/+; TAg+/- Cdh 11+/-; TAg+/- Cdh 11 -/-; TAg+/- Percent Tumor Area per Retinal Area at PND 84 % Total Tumor Area per Retina [pixels] C 30 25 K-W: p=0. 07; with moving that one sample, now it is showing a strong trend to significance 20 15 10 5 0 Figure 7 Cdh 11+/+; TAg+/- Cdh 11+/-; TAg+/- Cdh 11 -/-; TAg+/-
B A K-W: p=0. 003 800 600 400 200 % PCNA Cells Per Tumor Volume % PCNA cells / tumor volume PND 84 Tumor Volume / P 8 Average Tag Positive cell 1000 Boxplot of Tumor Volume at PND 84 from a Single PND 8 Cell 40 p=0. 113 35 30 25 20 15 10 5 0 0 Cdh 11 TAg+/- +/+; Cdh 11 TAg+/- +/-; Cdh 11 -/-; TAg+/- Cdh 11+/+; TAg+/- Cdh 11 -/-; TAg+/- C Number of Caspase-3 positive cells per tumor volume per eye Cell Death per Tumor Volume in Mouse Retinas 7. 00 E-03 Mann-Whitney Test: p=0. 04 6. 00 E-03 5. 00 E-03 4. 00 E-03 Can you change th and indicate the S means in the leg 3. 00 E-03 2. 00 E-03 1. 00 E-03 0. 00 E+00 Cdh 11+/+; TAg+/- Figure 8 Cdh 11 -/-; TAg+/-
A Cdh 11+/+ Cdh 11 -/- Chx-10 160 k. Da HPC-1 Cralbp Brd-U Cadherin-2 Supplementary Figure 1 B Cdh 11+/+ Cdh 11 -/- C Cdh 11+/+ Cdh 11 -/-
Number of Positively Stained Cells Hes 5 and Caspase-3 Positive cells in retinas of mice 80 p = 0. 35 70 60 CDH 11+/+; TAg+/- 50 CDH 11 -/-; TAg+/- 40 30 20 10 0 Hes-5 Supplementary Figure 2 Activated Caspase-3
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