50 Components Stock concentration Final concentration Template 125

ﻣﻴﻜﺮﻭﻟﻴﺘﺮ ﺍﻟﺤﺠﻢ ﺍﻟﻜﻠﻲ 50 ﺍﻟﻜﻤﻴﺎﺕ ﺍﻟﻤﻄﻠﻮﺑﺔ ﻟﺘﺤﻀﻴﺮ : ﻟﻠﺘﻔﺎﻋﻞ ﻫﻲ Components Stock concentration Final concentration Template 125 ng/μL 7. 5 ng/μL Primer F 200 ng/μL 4. 0 ng/μL Primer R 200 ng/μL 4. 0 ng/μL d. NTP mix 2. 0 m. M each 0. 2 m. M PCR buffer 10 X 1 X Mg. Cl 2 25 m. M 1. 5 m. M Taq polymerase 5 U/μL 0. 05 U/μL H 2 O

ﻣﻴﻜﺮﻭﻟﻴﺘﺮ ﺍﻟﺤﺠﻢ ﺍﻟﻜﻠﻲ 50 ﺍﻟﻜﻤﻴﺎﺕ ﺍﻟﻤﻄﻠﻮﺑﺔ ﻟﺘﺤﻀﻴﺮ : ﻟﻠﺘﻔﺎﻋﻞ ﻫﻲ Components Stock concentration Final concentration Template 125 ng/μL 7. 5 ng/μL 3 Primer F 200 ng/μL 4. 0 ng/μL 1 Primer R 200 ng/μL 4. 0 ng/μL 1 d. NTP mix 2. 0 m. M each 0. 2 m. M 5 PCR buffer 10 X 1 X 5 Mg. Cl 2 25 m. M 1. 5 m. M 3 Taq polymerase 5 U/μL 0. 05 U/μL H 2 O Volume μl 0. 5 31. 5

ﻣﻴﻜﺮﻭﻟﻴﺘﺮ ﺍﻟﺤﺠﻢ ﺍﻟﻜﻠﻲ ﻟﻠﺘﻔﺎﻋﻞ 20 ﺍﻟﻜﻤﻴﺎﺕ ﺍﻟﻤﻄﻠﻮﺑﺔ ﻟﺘﺤﻀﻴﺮ : ﻫﻲ Components Stock concentration Final concentration Template 125 ng/μL 7. 5 ng/μL Primer F 200 ng/μL 4. 0 ng/μL Primer R 200 ng/μL 4. 0 ng/μL d. NTP mix 2. 0 m. M each 200 μM PCR buffer 10 X 1 X Mg. Cl 2 25 m. M 1500 μM Taq polymerase 5 U/μL 0. 05 U/μL H 2 O

ﻣﻴﻜﺮﻭﻟﻴﺘﺮ ﺍﻟﺤﺠﻢ ﺍﻟﻜﻠﻲ ﻟﻠﺘﻔﺎﻋﻞ 20 ﺍﻟﻜﻤﻴﺎﺕ ﺍﻟﻤﻄﻠﻮﺑﺔ ﻟﺘﺤﻀﻴﺮ : ﻫﻲ Components Stock concentration Final concentration Volume (μL) Template 125 ng/μL 7. 5 ng/μL 1. 2 Primer F 200 ng/μL 4. 0 ng/μL 0. 4 Primer R 200 ng/μL 4. 0 ng/μL 0. 4 d. NTP mix 2. 0 m. M each 200 μM 2 PCR buffer 10 X 1 X 2 Mg. Cl 2 25 m. M 1500 μM 1. 2 Taq polymerase 5 U/μL 0. 05 U/μL 0. 2 H 2 O 12. 6