5 16 Gel Electrophoresis Ms Blalock Ms Hartsell
5 -16 Gel Electrophoresis Ms. Blalock, Ms. Hartsell, Mr. Luckman
Do Now What do restriction enzymes do to a DNA sample?
Restriction Enzymes Recognizes and cuts DNA only at a particular sequence of base pairs (ATGC)
Aim: How does Gel Electrophoresis help solve murder crimes? Agenda • • • Do Now Hook Activity 1: Introduction to Gel Electrophoresis Activity 2: Interactive Activity 3: Lab Report Part I – Background Information • Exit Ticket
Hook Video How can technology help solve murder mysteries? http: //vitalny. pbslearningmedia. org/asset/tdc 02 _vid_sheppard/
Gel Electrophoresis A technique used to show species are related to one another
Gel Electrophoresis Goal – Find a DNA match between two samples. Related organisms will show similar banding patterns because their DNA is the same
Gel Electrophoresis Goal – Find a DNA match between two samples. The DNA bands should have a similar pattern between identical species
Gel Electrophoresis Example – W = Blood at crime scene. X, Y, and Z are DNA samples of suspects
Gel Electrophoresis So how does this work?
Gel Electrophoresis So how does this work?
Step 1 – Obtain DNA samples
Step 2 – Add restriction Enzymes Restriction Enzymes split the DNA into particular lengths Example-- cuts at CC/GG Put a line where the enzyme would cut the DNA AGCTCCGGTCATGCCGGATGACTGATCCGGATA
Step 2 – Add restriction Enzymes Restriction Enzymes split the DNA into particular lengths Example-- cuts at CC/GG Put a line where the enzyme would cut the DNA AGCTCCGGTCATGCCGGATGACTGATCCGGATA
Step 2 – Add restriction Enzymes AGCTCCGGTCATGCCGGATGACTGATCCGGATA Separate pieces – based on length GGATA AGCTCC GGTCATGCC GGATGACTGATCC
Step 3 - DNA is placed in the wells DNA
Step 4 – Electricity is added - +
Step 5 – Observe Banding An electric current carries the DNA fragments through the gel, separating them according to size (smaller pieces of DNA are carried farther from the well than larger pieces) Large pieces GGATGACTGATCC Small pieces GGTCATGCC AGCTCC GGATA GGATGACTGATCC GGTCATGCC AGCTCC GGATA
Check for Understanding
Activity 2 Directions: 1. Go to www. fordhamarts. com 2. Living Environment 3. Unit 5 -Genetics 4. Interactive/Video 5. 5 -16 Gel Electrophoresis In groups, go through the interactive and answer the guiding questions in your notes ACTUALLY READ THE TEXT IN THE INTERACTIVE OR YOU WILL NOT LEARN http: //www. classzone. com/books/hs/ca/sc/bio_07/virtu al_labs/virtual. Labs. html
Activity 3 – Lab Introduction Directions: Read overview of lab report instructions • Over the next few days we will be working to solve a murder crime using Gel Electrophoresis. • Your end work product will be a typed lab report.
Activity 3 – Background Information • Look at an example background information section of a lab report • Look at the section of the rubric on background information What would you rate this example and why?
Activity 3 – Background Information Directions: Create your draft background information based on what you have learned today about Gel Electrophoresis. Create your draft on Google Drive 1. 2. 3. 4. 5. www. Google. com/drive Username: firstname. lastname@fhsaonline. org Password: ID number Click on “Create” -> Document Name the document “Firstname. Lastname. Gel. Electrophoresis. Lab 6. Use 12 font
Activity 3 – Finish Early? • Use the rubric to peer assess a neighbor’s background information
Exit Ticket
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