3 Materials and Methods v Materials Compound Y

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3. 실험재료 및 실험방법 Materials and Methods v Materials - Compound Y ( 410

3. 실험재료 및 실험방법 Materials and Methods v Materials - Compound Y ( 410 nm에서 최대흡광도의 물질) 10, 20, 40, 60 u. M Y compound in 100 m. M Tris-Cl buffer and Blank - Spectrophotometer v Methods 1) spectrophotometer의 wavelength를 410 nm로 설정 및 blank 측정 2) 농도가 주어진 4개의 compound Y 용액을 cuvette에 1 m. L 옮긴 후 spectrophotometer에서 O. D를 측정 3) 측정한 O. D 결과를 이용하여 Standard curve를 그림 4) compound Y의 몰 흡광계수를 구함

4. 보고서 작성 Results 1. Absorbance & Standard curve Y (u. M) OD 410

4. 보고서 작성 Results 1. Absorbance & Standard curve Y (u. M) OD 410 nm 0 10 20 40 60 3. Molar absorptivity e=

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