2018 2019 LETTUCE TZ PROFICIENCY PARTICIPANTS 172 PARTICIPANTS

2018 -2019 LETTUCE TZ PROFICIENCY

PARTICIPANTS • 172 PARTICIPANTS • • • 2 CSAAC 7 CSAAC/RST 2 CSA IN GERM 9 CSA IN PURITY AND GERM 12 CVT 1 CSAG/CVT 95 RST 14 RST/CSA IN PURITY AND GERM 30 NOT ACCREDITED

RESULTS • Average TZ was a 90% • Results ranged from an 18% to a 100% • 153 individuals were within one standard deviation • 9 individuals were within two standard deviations • Of these two are non accredited individuals • 10 individuals were outside two standard deviations • Of these four are non accredited individuals

NUMBER OF SEEDS • 5 INDIVIDUALS TESTED 400 SEEDS • 4 were within 1 standard deviation • 1 was within 2 standard deviations • 1 INDIVIDUAL TESTED 160 SEEDS • Was outside 2 standard deviations • 1 INDIVIDUAL TESTED 100 SEEDS • Was within 1 standard deviation • 165 INDIVIDUALS TESTED 200 SEEDS • 149 were within 1 standard deviation • 8 were within 2 standard deviations • 9 were outside 2 standard deviations • Rules require a minimum of 200 seeds be tested for a stand alone TZ Test

2018 -2019 LETTUCE TZ PROFICIENCY 66 212 88 105 116 124 132 181 190 199 207 215 30 4143 54 63 87 95 120 141 144 152 175 213 219 5 32 52 70 83 848689 93 97 123 128 142 166 174 176 184 192196 203 208 214218 3 343740 50 67 7477 94 98 108 114 122 130 140 157 193 201 202206 209 221 4 16 47 60 61 82 101 109 111 112 127 180 204 42 68 80 85 121 126 133 138 148 156 183 220 11 57 65 71 76 90 145 165 197 6 27 38 51 158 187 210 22 125 155 163 169 178 19 23 69 33 72 100 160 12 2 8 95 15 13 14 85 1 232 233 229 230 226 25 48 79 35 146 118 211 172 53 29 75 231235 237 239 242 238 241 7578 200 173 119 92 59 64 167 91 65 147 195 194 55 7 45 234 35 73 25 102 15 0 10 20 30 40 50 60 70 80 90 100 110 120 130 140 150 160 170 180 190 200 210 220 230 240 250

COMMENTS • Why a lettuce TZ? Germination test is only 7 days • Most seeds sprouted overnight. • Called sprouted viable • Sample showed necrosis • Difficult to interpret • Conducted germ test prior to TZ. Germ showed necrosis • Did not know necrosis was detectable with a TZ test

TZ Handbook for Lactuca sativa • Family Asteraceae Genera (II) •

PREPARATION

EVALUATION

DETECTING NECROSIS IN LETTUCE TZ • Consulted with TZ committee since no where in the TZ handbook does it state anything about determining necrosis in lettuce TZ. It warns of artifact damage and to ensure membranous covering is cut open. • Lettuce sample was necrotic but most of the necrotic lettuce stained normally and must be called viable. • Considered unviable • • • Any essential part of embryo unstained Radicle with unstained areas extending into the conducting tissue Cotyledon ½ or more unstained or unstained near the point of attachment Immature embryo Embryo broken or cracked Extensive bruising seen as dark red or gray