2013 14 Crop Development Centre Two Row Barley

2013 -14 Crop Development Centre Two. Row Barley Research Check-Off Project A. D. Beattie Crop Development Centre University of Saskatchewan, Saskatoon, SK WGRF Barley Technical Advisory Committee Meeting August 12, 2014

CDC Breeding Program Staff § 20 full-time staff § § 2 in grain quality lab 2 in malt quality lab 5 in molecular lab 11 in field lab § 3 summer staff § 5 graduate students

Barley Breeding Focus § Barley (2 -row) § § malting feed forage hulless food/malting

Barley Breeding Objectives § Agronomics § Yield § Lodging and shattering resistance § Maturity § Grain and Quality § § TWT, TKW, Plumpness – Uniformity Grain Protein Malt Quality Hull Adherance § Disease § FHB, spot blotch, net blotch, scald, smuts, stem rust

2014 CDC Crossing Block Category Hulless High Amylose Waxy Malt Percentage of Crosses 5% 9% 8% General Purpose 13% Malt Introductions 3 -Way FHB Scald Leaf Disease Septoria Ug 99 Craft Low Beta-glucan Yield 8% 9% 3% 9% 10% 2% 4% 11% 5% 8% Forage Total: 6% 100%

2014 Supported for Registration Entry Years CDC Copeland C Xena C AC Metcalfe C TR 11127 2 Yield (Kg/Ha) 5336 5599 5011 5472 Yield as % Met 106 111 100 109 Plump % 1000 KWt Protein G. E. Entry (>7/64”) (>6/64”) (g) (%) 4 m. L 8 m. L CDC Copeland 70. 3 93. 4 44. 6 11. 9 100 95 AC Metcalfe 64. 8 90. 7 41. 9 12. 7 99 90 83. 0 97. 4 46. 9 11. 8 100 94 TR 11127 Days to Head 58. 4 56. 3 56. 4 58. 0 Steepout (%) 43. 4 44. 2 44. 0 Plump % 1000 KWt Protein G. E. Steepout Entry (>7/64”) (>6/64”) (g) (%) 4 m. L 8 m. L (%) CDC Copeland 47. 1 88. 6 43. 0 10. 7 100 95 43. 8 AC Metcalfe 61. 9 92. 5 43. 1 11. 7 99 86 44. 0 79. 0 96. 2 48. 1 11. 0 99 87 43. 9 TR 11127 Days to Maturity 92. 7 92. 9 92. 4 93. 4 Height (cm) 85. 9 81. 5 83. 0 Lodging 3. 5 3. 2 3. 6 2. 2 Test Weight Kernel Weight % Plump (Kg/HL) (g/1000 k) (>6/64”) 63. 4 44. 2 87. 6 65. 6 47. 6 88. 8 64. 7 43. 1 86. 8 65. 5 47. 2 92. 5 F. Ext. (%) 80. 0 79. 9 81. 3 Sol. Protein (%) 4. 6 5. 0 5. 3 Ratio S/T 37. 7 39. 0 44. 6 DP (°L) 128 153 146 AA (D. U. ) 56. 5 82. 5 77. 4 BG (ppm) 88 87 56 Visc. (cps) 1. 46 1. 45 1. 43 FAN mg/L 169 197 203 Friab. (%) 81. 0 72. 4 81. 9 Peeled (%) 2. 4 3. 2 2. 5 F. Ext. (%) 80. 6 80. 8 81. 8 Sol. Protein (%) 4. 30 4. 24 5. 03 Ratio S/T 39. 7 36. 7 47. 2 DP (°L) 104 128 126 AA (D. U. ) 52. 8 69. 0 69. 6 BG (ppm) 96 124 79 Visc. (cps) 1. 44 1. 45 1. 42 FAN mg/L 156 166 194 Friab. (%) 86. 6 79. 2 92. 2 Peeled (%) 6. 5 8. 1 6. 2 § TR 11127 (SM 04261/TR 05285): § Good yield potential, strong § Se. Can § Collab comments: low β-glucan, good extract, low peeling, higher soluble protein and S/T.

2014 Supported for Registration Entry Years CDC Copeland C Xena C AC Metcalfe C TR 07921 2 Yield (Kg/Ha) 5742 6228 5498 5728 Plump % 1000 KWt Protein G. E. Entry (>6/64”) (g) (%) 4 m. L 8 m. L CDC Copeland 94. 1 43. 1 10. 2 97 96 AC Metcalfe 92. 8 42. 3 10. 7 98 96 94. 1 43. 5 10. 6 99 91 TR 07921 Yield as % Met 104 113 100 104 Steepout (%) 46. 6 46. 9 46. 6 Plump % 1000 KWt Protein G. E. Steepout Entry (>6/64”) (g) (%) 4 m. L 8 m. L (%) CDC Copeland 98. 1 49. 9 10. 9 99 98 43. 4 AC Metcalfe 97. 6 50. 0 11. 1 100 92 44. 0 98. 7 51. 6 11. 0 99 92 43. 3 TR 07921 Days to Head 59. 0 56. 6 58. 6 Days to Maturity 90. 0 90. 3 89. 2 89. 7 Height (cm) 87. 0 85. 6 85. 9 88. 2 Lodging 4. 5 3. 8 4. 2 5. 5 Test Weight Kernel Weight % Plump (Kg/HL) (g/1000 k) (>6/64”) 63. 3 43. 8 88. 0 66. 2 47. 2 89. 0 65. 2 42. 7 87. 8 63. 6 44. 5 88. 4 F. Ext. (%) 81. 1 81. 7 80. 6 Sol. Protein (%) 4. 62 4. 78 4. 58 Ratio S/T 45. 6 46. 7 44. 3 DP (°L) 105 123 98 AA (D. U. ) 52. 0 68. 0 50. 1 BG (ppm) 80 87 83 Visc. (cps) 1. 43 1. 42 1. 43 Friab. (%) 98. 4 99. 4 96. 4 Peeled (%) 5. 7 4. 8 2. 2 F. Ext. (%) 81. 1 81. 3 80. 8 Sol. Protein (%) 4. 45 4. 74 4. 46 Ratio S/T 40. 3 42. 6 40. 4 DP (°L) 116 142 116 AA (D. U. ) 54. 4 73. 2 54. 0 BG (ppm) 77 80 82 Visc. (cps) 1. 45 1. 44 FAN mg/L 141. 5 154. 5 133. 0 Friab. (%) 88. 1 80. 6 82. 3 § TR 07921: (aka LOXless Copeland) § Canterra Seeds Peeled (%) 4. 6 3. 8 3. 2

2014 2 nd Year Collabs Entry CDC Copeland Xena AC Metcalfe TR 12135 TR 12137 TR 12810 Yield (Kg/Ha) 5681 5969 5357 6090 5831 5591 Yield as % Met 106 111 100 114 109 104 Days to Head 56. 3 53. 6 54. 0 55. 8 55. 5 55. 3 Days to Maturity 93. 3 92. 5 92. 6 93. 8 93. 0 92. 0 Height (cm) 86. 8 83. 1 83. 9 82. 5 86. 8 83. 5 Lodging 3. 7 3. 5 4. 1 2. 8 3. 7 3. 5 Test Weight Kernel Weight % Plump (Kg/HL) (g/1000 k) (>6/64”) 63. 7 45. 3 87. 6 65. 6 48. 3 88. 6 65. 2 44. 3 87. 7 64. 2 46. 8 91. 4 64. 9 42. 9 87. 1 64. 4 45. 5 90. 8 Plump % 1000 KWt Protein G. E. Steepout Entry (>7/64”) (>6/64”) (g) (%) 4 m. L 8 m. L (%) CDC Copeland 49. 1 94. 4 44. 0 11. 2 99 91 43. 4 AC Metcalfe 57. 0 93. 8 40. 0 11. 4 100 91 43. 6 TR 12135 79. 6 98. 0 46. 3 10. 5 100 90 43. 7 TR 12137 52. 7 96. 2 41. 6 10. 9 100 96 44. 0 TR 12810 74. 7 95. 5 45. 0 11. 0 99 93 44. 6 F. Ext. (%) 81. 3 82. 3 83. 3 81. 5 82. 9 Sol. Protein (%) 5. 08 5. 09 5. 02 4. 83 5. 25 Ratio S/T 47. 2 46. 7 51. 0 46. 0 50. 7 DP (°L) 132 144 150 117 151 AA (D. U. ) 59. 4 79. 2 70. 1 59. 1 77. 2 BG (ppm) 91 105 44 40 36 Visc. (cps) 1. 44 1. 46 1. 43 1. 42 1. 44 FAN mg/L 193 207 200 203 213 Friab. (%) 89. 3 83. 3 95. 8 92. 8 91. 0 Peeled (%) 7. 1 10. 0 6. 8 5. 8 6. 7 Plump % 1000 KWt Protein G. E. Steepout Entry (>7/64”) (>6/64”) (g) (%) 4 m. L 8 m. L (%) CDC Copeland 47. 1 88. 6 43. 0 10. 7 100 95 43. 8 AC Metcalfe 61. 9 92. 5 43. 1 11. 7 99 86 44. 0 TR 12135 70. 3 95. 5 45. 6 10. 9 99 92 44. 3 TR 12137 58. 1 95. 3 42. 2 10. 5 99 89 43. 6 TR 12810 62. 5 93. 9 42. 6 11. 7 96 70 45. 9 F. Ext. (%) 80. 6 80. 8 81. 1 80. 7 80. 4 Sol. Protein (%) 4. 30 4. 24 3. 99 4. 30 4. 63 Ratio S/T 39. 7 36. 7 37. 2 40. 4 39. 3 DP (°L) 104 128 134 96 134 AA (D. U. ) 52. 8 69. 0 63. 9 51. 9 66. 8 BG (ppm) 96 124 110 49 130 Visc. (cps) 1. 44 1. 45 1. 42 1. 46 FAN mg/L 156 162 171 Friab. (%) 86. 6 79. 2 91. 7 91. 2 80. 2 Peeled (%) 6. 5 8. 1 3. 6 7. 4 5. 3 § TR 12135 (TR 04280/SM 04261) § Strong, Good Yield potential, SFNB R, Rpg 1, Un 8 § TR 12137 (SM 04640 s/SM 04589): Craft potential? ? , Rpg 1 § TR 12810 (KB 3 X 2 N 22 P 2/SM 03399 s): SFNB R, surface smut R

2014 2 nd Year 2 RCoop Entry Years CDC Copeland C Xena C AC Metcalfe C TR 13144 1 TR 13145 1 TR 13812 1 Yield (Kg/Ha) 6504 6884 6001 6752 6883 6630 Yield as % Met 108 115 100 113 115 110 Days to Head 53. 4 51. 0 51. 7 52. 6 52. 0 52. 9 Plump % 1000 KWt Protein G. E. Steepout Entry (>7/64”) (>6/64”) (g) (%) 4 m. L 8 m. L (%) CDC Copeland 49. 1 94. 4 44. 0 11. 2 99 91 43. 4 AC Metcalfe 57. 0 93. 8 40. 0 11. 4 100 91 43. 6 TR 13144 64. 1 96. 2 44. 6 10. 5 99 96 43. 6 TR 13812 63. 0 95. 3 44. 0 10. 9 100 88 43. 2 Days to Maturity 96. 1 94. 9 95. 0 96. 4 95. 6 F. Ext. (%) 81. 3 82. 6 82. 5 Height (cm) 88. 5 83. 3 85. 2 85. 0 84. 8 Sol. Protein (%) 5. 08 5. 09 5. 21 4. 77 Lodging 3. 4 3. 2 4. 7 3. 7 1. 9 2. 6 Ratio S/T 47. 2 46. 7 51. 0 45. 1 Test Weight Kernel Weight % Plump (Kg/HL) (g/1000 k) (>6/64”) 65. 7 48. 4 92. 2 68. 0 51. 0 92. 9 67. 3 46. 7 91. 6 67. 2 48. 9 93. 8 67. 0 50. 1 92. 7 67. 1 48. 1 93. 4 DP (°L) 132 144 137 148 AA (D. U. ) 59. 4 79. 2 76. 6 77. 8 BG (ppm) 91 105 43 91 Visc. (cps) 1. 44 1. 46 1. 43 1. 44 § TR 13144 (TR 08116/Major): § Good Yield Potential, Weak? § TR 13145 (CDC Austenson/TR 06390): § Good Yield Potential, Strong § TR 13812 (TR 03375/TR 07921//CDC Reserve): § Improved Yield over CDC Polar. Star, Strong FAN mg/L 193 207 217 190 Friab. (%) 89. 3 83. 3 95. 1 91. 5 Peeled (%) 7. 1 10. 0 4. 5 8. 3

2014 1 st Year 2 RCoop CDC# SM 110075 SM 110138 SM 111546 SM 110384 SM 110530 Pedigree TR 07113/Major TR 08116/Major SSP 06005/CDC Kindersley TR 06292/TR 07110 TR 08116/SB 050739 2013 Test# SBYT#2 -22 SBYT#2 -27 SBYT#2 -30 SBYT#2 -36 SBYT#3 -34 Type Malt Malt 2014 Coop# TR 14146 TR 14147 TR 14148 TR 14149 TR 14150 § TR 14146: Good YLD, strg, shrt, G grain, G protein, Enz<Met and >Cop, <BG, >Ext, VG leaf dis § TR 14147: Good YLD, ok strength, shrtr, G Grain, G protein, >Enz, <BG, >Ext, § TR 14148: Ok Yield YLD, ok strength, G Grain, G Protein, Cop Enz, <BG, VG leaf dis § TR 14149: Good YLD, strg, ltr, G Grain, OK Protein, >Enz, ok BG, >FAN, >Ext § TR 14150: Good YLD, strg, earlier, shrtr, G Grain, Cop Enz, <BG, >FAN, scald R

2014 Supported for Registration Yield Entry Yield as Days to Height D. Test Weight C. Test Weight Kernel Weight % Plump BG AEV (Kg/Ha) % Mc. G Head Maturity (cm) Lodging (Kg/HL) (g/1000 k) (>6/64”) (%) (cps) AC Metcalfe 5845 104. 7 56. 2 94. 6 86. 6 4. 3 63. 8 65. 6 43. 8 94. 4 4. 6 10. 7 CDC Mc. Gwire 5667 100. 0 58. 3 97. 2 89. 4 3. 2 73. 6 79. 0 39. 7 85. 7 5. 0 16. 2 CDC Rattan 5127 90. 6 59. 6 96. 5 80. 9 2. 9 70. 7 78. 4 37. 6 89. 3 7. 9 65. 0 HB 12321 5325 95. 4 56. 7 90. 4 3. 2 73. 1 78. 5 37. 8 79. 2 6. 8 16. 2 § HB 12321 (SH 060751/CDC Mc. Gwire): § High amylose, higher beta-glucan § MR to SFNB, covered smut, CRR, R to stem rust § Market research in progress

2014 2 nd Year HBCoop D. Test Weight C. Test Weight Kernel Weight % Plump Yield as Days to Height BG AEV Entry (Kg/Ha) % Mc. G Head Maturity (cm) Lodging (Kg/HL) (g/1000 k) (>6/64”) (%) (cps) AC Metcalfe CDC Mc. Gwire 6359. 1 6019. 2 107. 5 100. 0 50. 8 53. 6 96. 2 99. 0 89. 4 92. 6 5. 1 3. 6 64. 4 74. 8 67. 9 80. 1 46. 7 42. 3 95. 8 90. 9 4. 62 5. 06 7. 5 13. 5 CDC Rattan HB 13324 5531. 1 6469. 8 91. 0 109. 2 54. 2 53. 7 98. 6 83. 8 87. 9 3. 5 3. 7 72. 1 74. 1 80. 3 39. 8 41. 9 92. 4 93. 4 7. 92 7. 81 68. 2 29. 4 § HB 13324 (SH 05256/CDC Austenson) § waxy § Rpg 1, Un 8, R to covered smut § MR to spot blotch

2014 1 st Year HBCoop § § CDC# Pedigree 2013 Test# Type 2014 Coop# SH 110547 HB 08301/TR 08115 2013 SBYT#1 -11 Malt HB 14326 SH 110601 CDC Ex. Plus/CDC Meredith//SM 06031 2013 SBYT#1 -14 Malt HB 14327 SH 110300 SH 060882/CDC Mc. Gwire 2013 SBYT#1 -23 High Amylose HB 14328 SH 110358 HB 394/CDC Austenson 2013 SBYT#1 -35 Feed HB 14329 HB 14326: YLD=Clear, >thresh, G protein, VG Q, G leaf dis HB 14327: YLD>Clear, strgr, >thresh, G protein, VG Quality HB 14328: YLD>Hilose, wk? , G thresh, amylose>Hilose HB 14329: >>YLD, >thresh, G leaf dis R, cov smut R

Equipment Investments § Malt quality evaluation § protein, friability, BGase, LD, AA, DP, FAN, extract § 4, 189 malted samples in 2013 -14 § 20, 101 analyses in 2013 -14

Marker-Assisted Selection § Un 8, Rpg 1, LOX-1, QRpt 6, protein (6 H) § ~ 9, 200 data points in 2013 -14

Barley Research Funding § Saskatchewan ADF projects § True loose smut marker development § FHB evaluation § Molson’s Canada/Canada Malting project § Low dimethyl sulfide precursor germplasm screening § ACIDF/Syngenta project § Crop inputs and varietal response

Barley Research Funding § AAFC Growing Forward 2 § § § 2013 -18 Expanded breeding (malting, feed, food) Scald and septoria mapping/backcrossing Association mapping/genomic selection Variety ID

True Loose Smut (Un 8) Gene Cloning § Caused by Ustilago nuda (Jens. ) Rostr. § Common seed-borne disease in the prairie provinces of Canada (50 -70% of barley fields) § Larger issue within organic production

True Loose Smut (Un 8) Gene Cloning Bd Os Sb Plant Cell 2011; 23: 1249 -1263

True Loose Smut (Un 8) Gene Cloning

True Loose Smut (Un 8) Gene Cloning

True Loose Smut (Un 8) Gene Cloning

Crop Inputs Trial Breeding Program Lines AAFC-Brandon TR 12225, TR 13231, AAC Synergy, AC Metcalfe AARD-Lacombe J 02039009, J 02033006, J 01073006, J 01039003 CDC TR 10124, TR 11127, TR 12135, TR 12137, TR 13140, TR 13142, CDC Meredith § Seed treatments § None § Cruiser Maxx Vibrance + Proseed § Axial Xtreme + MCPA § Quilt (½ rate with herbicide, full rate at flag leaf) § Yield, physical seed quality and malting quality

Crop Inputs Trial § For yield, maturity, plumpness at 3 locations § Variety was significantly different § Treatment and Variety x Treatment were not significantly different § Malt quality data still to be analyzed

Other Program Outputs § In the past year: § § § § § 5 published articles in refereed journals 2 published articles in non-refereed journals 6 manuscript reviews 10 poster presentations 1 class taught (PLSC 411) 1 undergraduate thesis project (PLSC 494. 6) 7 producer/industry field tours 3 producer presentations >30 extension/business meetings

Acknowledgements § WGRF § ADF § Se. Can § Busch Agricultural Resources Inc. § Sapporo/Prairie Malt Ltd.