2008 2013 The Human Microbiome Project HMP the

Международные проекты • 2008 -2013 – проект «Микробиом человека» (The Human Microbiome Project, HMP) the human microbiome includes around 100 trillion bacterial cells • 2014 – проект «Интеграционный микробиом человека» (The Integrative Human Microbiome Project, i. HMP)

National Institutes of Health (NIH), USA Human Microbiome Project Proctor L. M. The Human Microbiome Project in 2011 and beyond. Cell Host Microbe. 2011; 10(4): 28791. doi: 10. 1016/j. chom. 2011. 10. 001.

NIH Human Microbiome Project 2 19% Микроорганизм Количество проектов Pseudomonas spp. 19 4 Achromobacter spp. 7 2 Burkholderia spp. 1 Body Subsite All projects Lower Respiratory 150 Nose 20 Nasopharynx Sinus Beck J. M. The Microbiome of the Lung. Transl Res. 2012 Oct; 160(4): 258– 266. Cui L. The Microbiome and the Lung. Ann Am Thorac Soc. 2014 Aug; 11(Suppl 4): S 227–S 232.

Zakharkina T, Heinzel E, Koczulla RA, Greulich T, Rentz K, Pauling JK, Baumbach J, Herrmann M, Grünewald C, Dienemann H, von Müller L, Bals R. Analysis of the airway microbiota of healthy individuals and patients with chronic obstructive pulmonary disease by TRFLP and clone sequencing. PLo. S One. 2013; 8(7): e 68302. doi: 10. 1371/journal. pone. 0068302. Print 2013. Figure A phylogenetic tree obtained from sequencing analysis illustrates the standard microbiota present in healthy lungs containing 75 leafs.

Микробиота респираторного тракта детей до 21 месяца с диагнозом МВ Madan JC et al. Serial analysis of the gut and respiratory microbiome in cystic fibrosis in infancy: interaction between intestinal and respiratory tracts and impact of nutritional exposures. MBio. 2012; 3(4). pii: e 00251 -12. doi: 10. 1128/m. Bio. 00251 -12

Анализ бактериома образцов детей (трахеальный аспират, мокрота) P 1 100% P 2 P 3 P 4 P 5 P 6 P 7 P 8 90% 80% TM 7 70% Tenericutes 60% Proteobacteria 50% Fusobacteria 40% Firmicutes 30% Cyanobacteria 20% Bacteroidetes 10% Actinobacteria [Thermi] B. cenocepacia ST 878, L. mirabilis 12 M 1 14 л 2 м 17 л 4 м 31 29 84 11 л 1 K B. cenocepacia ST 709 7 N 26 N 14 4 г 9 м 4 г 10 м 21 N 25 17 N 27 N 18 4 г 3 м 27 18 N 7 2 г 8 м 3 г 3 м 6 29 N 2 г 1 м 20 25 N 22 м 27 N 27 1 14 м N 23 26 N 22 6 м 9 0% P. aeruginosa : 0, 04; 10; 90%

Образцы пациента P 8 100% 90% 80% TM 7 70% Proteobacteria 60% Fusobacteria 50% Firmicutes 40% Cyanobacteria 30% Bacteroidetes 20% Actinobacteria 10% [Thermi] 0% 299 14 л 2 м 311 14 л 4 м 1 K 12 N 17 л 4 м(N) 1 K 12 M 17 л 4 м (S) 3 K 10 N 17 л 9 м(N) 3 K 10 Z 17 л 9 м(O)

Образцы пациента P 6 100% 90% 80% TM 7 70% Tenericutes 60% Proteobacteria 50% Fusobacteria 40% Firmicutes 30% Cyanobacteria 20% Bacteroidetes 10% Actinobacteria 0% 25 N 21(A) A 1 26 N 14(A) A 2 26 N 17(N) N 26 N 18(Z) A 3 Burkholderia 8%, Ralstonia 3%, Comamonadaceae 3%, Pseudomonas 4%

Firmicutes 100% 80% 60% 40% 20% 0% P 1 S P 2 S P 3 S 1 P 3 S 2 P 3 N P 4 S 1 P 4 S 2 P 4 N P 5 S 1 P 5 S 2 P 5 S 3 P 5 S 4 P 5 S 5 P 5 S 6 Proteobacteria 120% 100% 80% 60% 40% 20% 0% P 1 S P 2 S P 3 S 1 P 3 S 2 P 3 N P 4 S 1 P 4 S 2 P 4 N P 5 S 1 P 5 S 2 P 5 S 3 P 5 S 4 P 5 S 5 P 5 S 6

Сравнение микроорганизмов верхнечелюстных пазух и мокроты 1 B. multivorans ST 712 2 A. ruhlandii ST 261 3 B. multivorans ST 835 4 P. aeruginosa, B. cenocepacia ST 709, Candida lusitaniae 5 P. aeruginosa 6 A. ruhlandii ST 36, B. cenocepacia ST 709, P. aeruginosa 1 B. multivorans ST 712 2 A. ruhlandii ST 261 3 Streptococcus constellatus , B. multivorans ST 835 4 Streptococcus spp. , B. cenocepacia ST 709, P. aeruginosa, Candida lusitaniae 5 P. aeruginosa, A. ruhlandii ST 261 6 B. cenocepacia ST 709, P. aeruginosa