11 th Conference on Retroviruses and Opportunistic Infections

11 th Conference on Retroviruses and Opportunistic Infections, February 8 -11, 2004; San Francisco, CA 4 The Effect of Atazanavir vs. Lopinavir/Ritonavir on Insulin-Stimulated Glucose Disposal Rate in Healthy Subjects MA Noor 1, DM Grasela 1, RA Parker 1, U Chaudhari 1, D Tackett 1, H Uderman 1, A Currie 1, S Agarwala 1, C Grunfeld 2, MF Giordano 3, SL Hodder 1, FT Fiedorek 1, E O’Mara 1 Poster # 702 Bristol-Myers Squibb Pharmaceutical Research Institute, 1 Princeton NJ, and 3 Wallingford CT, 2 University of California San Francisco, CA, USA INTRODUCTION RESULTS Hyperinsulinemic Euglycemic Clamp: The clamp was performed as described by De. Fronzo et al (7). Insulin was administered as a primed continuous intravenous infusion, followed by a constant infusion at the rate of 40 U/m 2 min. A variable infusion of 20% dextrose was used to maintain plasma glucose concentration at ~75 mg/dl. Steady state glucose disposal rate was calculated between 60 to 180 minutes. Statistical Analyses: The effect of treatment was compared by analysis of variance (ANOVA). The factors in the analysis of variance were sequence, subject within sequence, period and treatment. Point estimates and 95% confidence intervals were constructed for treatment differences (ATV vs. LPV/r, ATV vs. placebo, LPV/r vs. placebo). All data are adjusted mean SE unless stated otherwise. Treatment A: ATV 400 mg QD + LPV/r BID Placebo (N=10) Treatment B: LPV/r 400/100 mg BID + ATV QD Placebo (N=10) Treatment C: ATV QD Placebo + A OR B 1 -5 or (N=10) CLAMP (180 min) A + PK Profile A 6 WASHOUT 7 days or or 14 C + PK Profile BMI (kg/m 2) 24. 0 2. 4 Fasting Plasma Glucose (mg/dl) 78. 0 4. 4 Fasting Serum Insulin ( IU/m. L) 6. 3 3. 3 Total Cholesterol (mg/dl) HDL Cholesterol (mg/dl) LDL Cholesterol (mg/dl) Fasting Triglycerides (mg/dl) B 19 n LPV/r administered in fasted state on Day 6 ATV administered in fasted state on Day 6 Figure 3 a: Insulin Levels by Treatment During Euglycemic Clamp Figure 3 b: Glucose Levels By Treatment During Euglycemic Clamp 176 15 52 5 102 10 108 25 n Steady State n Figure 4 a: Mean Glucose Disposal Rate per Unit of Insulin Figure 4 b: Glucose Oxidation and Glycogen Storage Rates Analysis by Treatment P=0. 010 6 12. 0 4. 00 3. 85 9. 80± 0. 84 9. 88± 0. 84 10. 0 7. 52± 0. 84 LPV/r (n=20) 8. 0 ATV (n=20) 6. 0 Placebo (n=20) n P=0. 003 P=N. S. 4 P=N. S. 3 LPV/r (n=20) 2. 54 ATV (n=20) Placebo (n=20) 1. 66 1. 72 1. 75 2 4. 0 2. 0 1 0. 0 0 Glucose Oxidation Rate Glycogen Storage Rate Figure 5: Effect of ATV vs. LPV/r on Insulin Sensitivity Figure 6: Lipid and Lipoproteins Prior to Clamp (Day 6) P< 0. 001 250 50 30 Insulin-Stimulated Glucose Disposal Rate per unit of Insulin (mg/Kg*min per U/m. L) Glycogen Storage Rate (mg/Kg*min) LPV/r (n=20) + 4% -10 ATV (n=20) P=N. S. 10 200 P< 0. 001 150 91 91 97 -30 -50 - 24% P=0. 008 - 34% P=0. 006 0 TC LDL REFERENCES 1. Dube MP et al. Lancet 1997; 350: 713 -4. 2. Grinspoon S. . Clin Infect Dis. 2003; 37 Suppl 2: S 85 -90. 3. 3. Noor MA, et al AIDS. 2001 May 4; 15(7): F 11 -8. 4. Noor MA, et al AIDS. 2002 Mar 29; 16(5): F 1 -8. 5. Murata H et al. J Biol Chem. 2000 Jul 7; 275(27): 20251 -4. 6. Wang S, et al. Antiviral Therapy 2003; 8: L 36. 7. De. Fronzo RA, et al 1979; 237: E 214 -23. 8. G. A. Lee, et al 10 th Conference on Retroviruses and Opportunistic Infections, Boston, MA 2003, Abstract No. 748. Although we have studied acute effects in healthy men, long-term results from clinical trials indicate that the extent and severity of metabolic complications are compounded by HIV and occur regardless of gender, suggesting clinically important adverse effects of ARV therapy on insulin sensitivity across diverse patient groups. Atazanavir (ATV) did not affect insulin sensitivity and had no effect on insulin-mediated glucose disposal rates as measured by the gold-standard glucose clamp technique. In contrast, lopinavir/ritonavir (LPV/r) induced significant insulin resistance when compared to both placebo-treated and ATVtreated subjects. LPV/r reduced the mean glucose disposal rate by 23% relative to ATV and by 24% relative to placebo. n ATV had no effect on the mean glycogen storage rate. In contrast, LPV/r significantly reduced the mean glycogen storage rate by 36% relative to ATV and 34% relative to placebo. n ATV did not significantly increase fasting triglycerides. In contrast, LPV/r significantly increased the mean fasting triglycerides by 43% compared to placebo and by 35% compared to ATV. n Our findings are consistent with in vitro studies of glucose uptake and confirm the favorable clinical metabolic profile of ATV. n LPV/r ATV Placebo 42 50 Our findings are in contrast to those by Lee et al who found little or no decrease in insulin-stimulated glucose disposal after 4 weeks of treatment with LPV/r in healthy volunteers (8). However, in that study insulin resistance was detected by oral glucose tolerance testing. In addition, Lee et al also observed significant increases in serum concentrations of free fatty acids and triglycerides, both known to be associated with insulin resistance. This work was performed at the Clinical Pharmacology Unit of Bristol-Myers Squibb Company. The authors thank Bruce Oliver, Marylou Bourgeois, R. N. , and the CPU nursing staff for technical assistance. Special thanks to Melissa Mc. Manus, Pharm. D. , for editorial assistance. P=NS 131124 100 < 1% P=N. S. 177 171 165 159 Plasma concentrations of PI drugs are highly variable as are individual subjects’ sensitivity to insulin. We measured glucose disposal rates near peak of plasma concentration for lopinavir and atazanavir at comparable insulin levels. ACKNOWLEDGMENTS CONCLUSION P=0. 006 P=0. 008 35 (range 19 -49) C CLAMP C Day LPV/r BID Placebo Mean wild-type EC 50 =4 ng/m. L 100% 76. 4 9. 9 Insulin-stimulated glucose disposal rates were consistently lower in individuals on LPV/r compared to both placebo and ATV. The magnitude of this effect was ~24% and within range of the effect previously reported on IDV (4, 5). The decrease is comparable in magnitude to total hepatic glucose production in fasted healthy individuals (~ 2 mg/kg/min). LPV Mean EC 50 =17 ng/m. L Study Population N= 30 Weight (kg) n (n=20) Table 1: Baseline Characteristics Median Age (years) Figure 1: Study Design Consistent with in vitro observations, we found that ATV did not have an effect on glucose disposal while LPV/r administration resulted in insulin resistance when compared to both ATV and placebo. This finding confirms the favorable profile of ATV on glucose metabolism observed in clinical trials. (n=20) 5 Male n Clamp mg/Kg/min Study Design: This was a randomized, double-blind, placebocontrolled, 3 -treatment, 2 -period cross-over study. All subjects were admitted to the Bristol-Myers Squibb Clinical Pharmacology Unit (CPU) as inpatients for 6 consecutive days. Subjects were randomly assigned to receive atazanavir (Reyataz Bristol-Myers Squibb, Princeton, NJ) 400 mg daily, lopinavir/ritonavir (Kaletra , Abbot Laboratories, Abbott Park, IL) 400/100 mg twice daily, or matching placebo. On the morning of day 6, a glucose clamp was performed. Treatment was discontinued on day 7 and the subjects were discharged from the CPU. Two week later subjects returned at which time they were randomly crossed over to an alternate treatment arm for repeat studies(Fig 1). DISCUSSION Figure 2 b: Pharmacokinetic Profiles of LPV and RTV During Euglycemic Clamp mg/dl After giving informed consent, healthy HIV- seronegative volunteers enrolled into the study. We excluded subjects with body mass index >30 kg/m 2, serum total cholesterol > 240 mg/dl triglycerides > 200 mg/dl, fasting glucose > 126 mg/dl , serum aspartate or alanine aminotransferases >50 U/l and creatinine >1. 4 mg/dl. Figure 2 a: Pharmacokinetic Profile of ATV During Euglycemic Clamp mg/Kg*min per micro. IU/ml METHODS Thirty six subjects were enrolled and 30 completed the study. Five subjects did not complete the study due to clamp procedure technical difficulties; one subject failed to return for repeat studies. All subjects were male; 14 were Caucasian, 9 African-American and 7 Hispanic/Latino. Baseline characteristics are listed in (Table 1). There were no adverse clinical or laboratory events. The clamp procedure was performed near peak plasma concentrations for each drug (Fig 2 a and Fig 2 b). A steady-state insulin level of 65 IU/ml was achieved after 30 minutes (Fig 3 a) and glucose was clamped at 75 mg/dl (Fig 3 b). The adjusted mean values of insulin-stimulated glucose disposal per unit of insulin (M/I) for LPV/r, ATV are shown (Fig 4 a). The difference in M/I between the ATV and LPV/r groups was statistically significant (2. 28 with 95% CI: 0. 58, 3. 97; p=0. 010). The difference in M/I between the LPV/r and placebo groups was also statistically significant (-2. 35 with 95% CI: -4. 05, -0. 66; p=0. 008). The difference in M/I between the ATV and placebo group was not statistically significant. The adjusted means of non-oxidative component of glucose disposal (glycogen storage) for individuals receiving LPV/r, ATV and placebo are shown (Fig 4 b). There were statistically significant differences in glycogen storage rate between the ATV and LPV/r groups (1. 46 with 95% CI: 0. 55, 2. 36; p=0. 003) and between the LPV/r and placebo groups (-1. 31 with 95% CI: -2. 22, -0. 40; p=0. 006). The difference in glycogen storage rates between the ATV and placebo groups was not statistically significant. Percentage changes relative to placebo are shown in Figure 5. The adjusted means of fasting lipids for LPV/r, ATV and placebo are shown in Fig 6. There were statistically significant differences in fasting triglycerides between the LPV/r and ATV groups (-46 mg/dl, p<0. 001) and between the LPV/r and placebo groups (+53 mg/dl, p<0. 001). The different in fasting triglycerides between the ATV and placebo groups was not statistically significant. % Change Relative to Placebo Treatment with some HIV protease inhibitors (PI) has been associated with insulin resistance, hyperglycemia, and development of diabetes mellitus (1, 2). Induction of insulin resistance by a PI precedes any significant changes in lipids, lipoproteins, or body composition (3) and can be detected after a single dose of indinavir (IDV) in healthy volunteers(4). In vitro studies suggest blockade of the insulin-regulated glucose transporter, GLUT-4, as a possible mechanism for PI-associated insulin resistance (5). Atazanavir (ATV) is a new PI that, unlike IDV, lopinavir (LPV), and ritonavir (RTV), does not block GLUT 4 activity (6). This studies assesses insulin-stimulated glucose disposal and glycogen storage rates in healthy volunteers taking ATV compared to those taking lopinavir/ritonavir (LPV/r) or placebo. R E S U L T S (Cont’d) Mustafa A. Noor, M. D. Phone: (609) 818 -4364/ 897 -5483 Fax: (609)897 -6068 E-mail: mustafa. noor@bms. com 42 40 HDL TG Data presented as mean Standard Deviation, unless stated otherwise 04 -005