10 m Human height Length of some nerve
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10 m Human height Length of some nerve and muscle cells 0. 1 m Chicken egg 1 cm 100 µm 10 µm Most plant and animal cells Nucleus Most bacteria 1 µm 100 nm 1 nm 0. 1 nm Mitochondrion Smallest bacteria Viruses Ribosomes Proteins Lipids Small molecules Atoms Electron microscope 1 mm Frog egg Light microscope 1 m Unaided eye Fig. 6 -2
Fig. 6 -8 Surface area increases while total volume remains constant 5 1 1 Total surface area [Sum of the surface areas (height width) of all boxes sides number of boxes] Total volume [height width length number of boxes] Surface-to-volume (S-to-V) ratio [surface area ÷ volume] 6 150 750 1 125 6 1. 2 6
Fig. 6 -6 Fimbriae Nucleoid Ribosomes Plasma membrane Bacterial chromosome (a) A typical rod-shaped bacterium Cell wall Capsule Flagella 0. 5 µm (b) A thin section through the bacterium Bacillus coagulans (TEM)
Fig. 6 -9 a ENDOPLASMIC RETICULUM (ER) Flagellum Rough ER Smooth ER Nuclear envelope NUCLEUS Nucleolus Chromatin Centrosome Plasma membrane CYTOSKELETON: Microfilaments Intermediate filaments Microtubules Ribosomes Microvilli Golgi apparatus Peroxisome Mitochondrion Lysosome
Fig. 6 -9 b NUCLEUS Nuclear envelope Nucleolus Chromatin Rough endoplasmic reticulum Smooth endoplasmic reticulum Ribosomes Central vacuole Golgi apparatus Microfilaments Intermediate filaments Microtubules Mitochondrion Peroxisome Chloroplast Plasma membrane Cell wall Wall of adjacent cell Plasmodesmata CYTOSKELETON
Fig. 6 -20 Microtubule 0. 25 µm Microfilaments
Table 6 -1 10 µm Column of tubulin dimers Keratin proteins Actin subunit Fibrous subunit (keratins coiled together) 25 nm 7 nm Tubulin dimer 10 µm 8– 12 nm
Fig. 6 -32 Tight junctions prevent fluid from moving across a layer of cells 0. 5 µm Tight junction Intermediate filaments Desmosome Gap junctions Space between cells Plasma membranes of adjacent cells Desmosome 1 µm Extracellular matrix Gap junction 0. 1 µm
Fig. 6 -3 ab TECHNIQUE RESULTS (a) Brightfield (unstained specimen) 50 µm (b) Brightfield (stained specimen)
Fig. 6 -3 cd TECHNIQUE (c) Phase-contrast (d) Differential-interferencecontrast (Nomarski) RESULTS
Fig. 6 -3 e TECHNIQUE RESULTS (e) Fluorescence 50 µm
Fig. 6 -4 TECHNIQUE (a) Scanning electron microscopy (SEM) RESULTS Cilia 1 µm (b) Transmission electron Longitudinal Cross section of of cilium microscopy (TEM) 1 µm cilium
Fig. 6 -9 a ENDOPLASMIC RETICULUM (ER) Flagellum Rough ER Smooth ER Nuclear envelope NUCLEUS Nucleolus Chromatin Centrosome Plasma membrane CYTOSKELETON: Microfilaments Intermediate filaments Microtubules Ribosomes Microvilli Golgi apparatus Peroxisome Mitochondrion Lysosome
Fig. 6 -10 Nucleus 1 µm Nucleolus Chromatin Nuclear envelope: Inner membrane Outer membrane Nuclear pore Pore complex Surface of nuclear envelope Rough ER Ribosome 1 µm 0. 25 µm Close-up of nuclear envelope Pore complexes (TEM) Nuclear lamina (TEM)
Fig. 6 -12 Smooth ER Rough ER ER lumen Cisternae Ribosomes Transport vesicle Smooth ER Nuclear envelope Transitional ER Rough ER 200 nm
Fig. 6 -11 Cytosol Endoplasmic reticulum (ER) Free ribosomes Bound ribosomes Large subunit 0. 5 µm TEM showing ER and ribosomes Small subunit Diagram of a ribosome
Fig. 6 -12 Smooth ER Rough ER ER lumen Cisternae Ribosomes Transport vesicle Smooth ER Nuclear envelope Transitional ER Rough ER 200 nm
Fig. 6 -13 cis face (“receiving” side of Golgi apparatus) 0. 1 µm Cisternae trans face (“shipping” side of Golgi apparatus) TEM of Golgi apparatus
Fig. 6 -14 Nucleus 1 µm Vesicle containing two damaged organelles 1 µm Mitochondrion fragment Peroxisome fragment Lysosome Digestive enzymes Plasma membrane Lysosome Peroxisome Digestion Food vacuole Vesicle (a) Phagocytosis (b) Autophagy Mitochondrion Digestion
Fig. 6 -15 Central vacuole Cytosol Nucleus Central vacuole Cell wall Chloroplast 5 µm
Fig. 6 -16 -1 Nucleus Rough ER Smooth ER Plasma membrane
Fig. 6 -16 -2 Nucleus Rough ER Smooth ER cis Golgi trans Golgi Plasma membrane
Fig. 6 -16 -3 Nucleus Rough ER Smooth ER cis Golgi trans Golgi Plasma membrane
Fig. 6 -14 Nucleus 1 µm Vesicle containing two damaged organelles 1 µm Mitochondrion fragment Peroxisome fragment Lysosome Digestive enzymes Plasma membrane Lysosome Peroxisome Digestion Food vacuole Vesicle (a) Phagocytosis (b) Autophagy Mitochondrion Digestion
Fig. 6 -9 b NUCLEUS Nuclear envelope Nucleolus Chromatin Rough endoplasmic reticulum Smooth endoplasmic reticulum Ribosomes Central vacuole Golgi apparatus Microfilaments Intermediate filaments Microtubules Mitochondrion Peroxisome Chloroplast Plasma membrane Cell wall Wall of adjacent cell Plasmodesmata CYTOSKELETON
Fig. 6 -9 a ENDOPLASMIC RETICULUM (ER) Flagellum Rough ER Smooth ER Nuclear envelope NUCLEUS Nucleolus Chromatin Centrosome Plasma membrane CYTOSKELETON: Microfilaments Intermediate filaments Microtubules Ribosomes Microvilli Golgi apparatus Peroxisome Mitochondrion Lysosome
Fig. 6 -17 Intermembrane space Outer membrane Free ribosomes in the mitochondrial matrix Inner membrane Cristae Matrix 0. 1 µm
Fig. 6 -9 b NUCLEUS Nuclear envelope Nucleolus Chromatin Rough endoplasmic reticulum Smooth endoplasmic reticulum Ribosomes Central vacuole Golgi apparatus Microfilaments Intermediate filaments Microtubules Mitochondrion Peroxisome Chloroplast Plasma membrane Cell wall Wall of adjacent cell Plasmodesmata CYTOSKELETON
Fig. 6 -18 Ribosomes Stroma Inner and outer membranes Granum Thylakoid 1 µm
Fig. 6 -28 Secondary cell wall Primary cell wall Middle lamella 1 µm Central vacuole Cytosol Plasma membrane Plant cell walls Plasmodesmata
Fig. 6 -24 Outer microtubule doublet 0. 1 µm Dynein proteins Central microtubule Radial spoke Protein crosslinking outer doublets Microtubules Plasma membrane (b) Cross section of cilium Basal body 0. 5 µm (a) Longitudinal section of cilium 0. 1 µm Triplet (c) Cross section of basal body Plasma membrane
Fig. 6 -25 Microtubule doublets ATP Dynein protein (a) Effect of unrestrained dynein movement ATP Cross-linking proteins inside outer doublets Anchorage in cell (b) Effect of cross-linking proteins 1 3 2 (c) Wavelike motion
Fig. 6 -23 Direction of swimming (a) Motion of flagella 5 µm Direction of organism’s movement Power stroke Recovery stroke (b) Motion of cilia 15 µm
Fig. 6 -22 Centrosome Microtubule Centrioles 0. 25 µm Longitudinal section Microtubules Cross section of one centriole of the other centriole
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