1 Laboratory Safety Orientation for New Employees USDA

1 Laboratory Safety Orientation for New Employees USDA Agricultural Research Service Pacific West Area Date Updated: November 2007

2 Training Guides Return to the beginning of current module Return to the table of contents Replay the slide audio Reference to the information pamphlet Module 0: Introduction

3 Training Modules 1. Administrative Information 2. Laboratory Ergonomics 3. Laboratory Hazards and Safety 4. Chemical Exposure 5. Hazard Communication 6. Exposure Control Methods Video Presentation Module 0: Introduction

4 Training Modules 7. Chemical Management 8. Shipping and Transport 9. Hazardous Waste 10. Environmental Management System 11. Emergency Response Challenge Exam Module 0: Introduction

5 Course Objectives • Follow USDA safety and security policies • Practice good ergonomic work habits • Identify laboratory hazards and apply proper protective measures • Handle and store chemicals safely • Dispose of hazardous waste properly • Know what to do in the event of an emergency on-site Module 0: Introduction

7 Administrative Information Safety and Security Policies Roles and Responsibilities Module 1: Administrative Information

8 Safety and Security • Employees must wear ID badges • Do not hold open doors • Be mindful of pedestrians in the parking lot • Sign in and escort guests at all times • Brief guests on important information Module 1: Administrative Information

9 Roles and Responsibilities Employees • Properly use protective equipment and clothing • Promptly report accidents, injuries and releases to supervisors • Complete required training Supervisors and Managers • Ensure employees are properly trained • Provide employees access to health and safety documentation • Conduct general inspections of laboratories • Provide procedures for particularly hazardous operations Module 1: Administrative Information

10 Roles and Responsibilities Senior Management • Provide administrative management in program implementation • Ensure proper reporting, investigation and follow-up of incidents and accidents Safety Contact • Recommend goals to reduce accidents and incidents • Conduct inspections and audits • Help managers/supervisors implement policies and procedures • Investigate reports of unsafe/unhealthy conditions Module 1: Administrative Information

11 Module Checkpoint • Do your part to provide a safe working environment • Apply training to communicate effectively with your supervisor or manager Module 1: Administrative Information

13 Laboratory Ergonomics Musculoskeletal Disorders Ergonomic Pipetting Practices Reporting Injuries Module 2: Laboratory Ergonomics

14 Musculoskeletal Disorder (MSD) • Result as a function of: – Force – Frequency – Posture • Overuse of muscles and tendons • Lack of recovery time • Injuries develop gradually Module 2: Laboratory Ergonomics

15 Ergonomics at the Working Surfaces • Avoid excessive reaching with “Comfort Zone” – Elbows at about 90 degrees – Nominal reaching with the upper arm • • Keep footspaces clear of storage Interrupt repetitive motions with mini-breaks Interrupt static postures by stretching Use proper lifting techniques Module 2: Laboratory Ergonomics

16 Ergonomic Pipetting Practices • Keep pipettors in good mechanical condition • Service units that become difficult to operate • Avoid long durations of pipetting • Allow time for mini-breaks • If you feel discomfort when pipetting, inform your supervisor Module 2: Laboratory Ergonomics

17 Reporting Injuries • • Inform your supervisor and Human Resources department Helpful Injury Information – – – Location(s) of discomfort Type of discomfort (broad, sharp, tingling, cold, numbing) Recurrence (all the time, only at certain times) History of the injury Tasks Module 2: Laboratory Ergonomics

18 Module Checkpoint • • • Do your part to reduce your risk to ergonomic injuries Position materials and supplies to reduce reaching Take breaks when working for extended periods of time Keep your equipment well-maintained Report any discomfort from repetitive tasks to your supervisor Module 2: Laboratory Ergonomics

20 Laboratory Hazards and Safety Physical Hazards Protective Measures Module 3: Laboratory Hazards and Safety

21 Physical Hazards Agent Effect Heat and Fire Thermal Burns Cryogens Frostbite Ultraviolet Exposure Sunburn Explosion Projectile Injury (Chemical Reaction, • Cuts/Laceration Compessed Gases) • Impact/Bruising • Injection Noise Module 3: Laboratory Hazards and Safety Hearing Damage

22 Physical Hazards in the Laboratory • Sonicators and Machinery – Hearing protection (ear plugs or earmuffs) • Ultraviolet Light (direct) – Polycarbonate faceshield – Cover exposed skin Module 3: Laboratory Hazards and Safety

23 Ultraviolet Radiation Exposure Symptoms • Eyes: inflammation, dry or scratchy feeling – • Long-term exposure may cause corneal damage Skin: resembles mild sunburn – Long-term exposure may possibly cause melanoma Safety Practices • Use handheld units in biosafety cabinets • PPE is mandatory (especially for face/eyes) Module 3: Laboratory Hazards and Safety

24 Glass Under Pressure Sources of Pressurization: Enclosed environments in glass present an explosion or implosion hazard! • • • Changes in temperature Chemical reaction Direct application of a gas Laboratory Precautions: • • • Module 3: Laboratory Hazards and Safety Inspect glassware before and after use Note pressure rating Wrap glassware with tape

25 Physical Hazards in the Laboratory • Autoclaves – Heat and Steam – Heat-resistant gloves – Lab coat or apron • Cryogens (Liquid Nitrogen) – Thermal gloves – Lab coat – Faceshield Module 3: Laboratory Hazards and Safety

26 Module Interactive In the space provided, list the physical hazards you expect to encounter in your laboratory work. Next to each item, identify the personal protective equipment (PPE) that you will need to safely work with these hazards. Module 3: Laboratory Hazards and Safety

27 Module Checkpoint • • • Do your part to reduce your exposure to physical hazards in the lab. Identify work that poses a physical hazard. Use the protective equipment available to guard against these hazards. Module 3: Laboratory Hazards and Safety

29 Chemical Exposure Physical and Health Hazards Exposure Routes Toxicity Module 4: Chemical Exposure

30 Physical Hazards • Chemical Burns (acids and caustics) • Physical Burn (flammable/ combustible) • Explosion (chemical reaction or compressed gases) • Asphyxiation (lack of oxygen) Module 4: Chemical Exposure

31 Health Hazards • Irritant • Allergen/Sensitizer • Reproductive/Developmental Toxin • Carcinogen • Target Organ Effects • Chemical Asphyxiant Module 4: Chemical Exposure

32 Routes of Exposure • Inhalation • Absorption • Ingestion • Injection Module 4: Chemical Exposure

33 Pinholes compromise the permeability of the glove Fumes from volatile solvents can be breathed in Working with potentially contaminated gloves A cut from contaminated glass provides direct chemical contact Module 4: Chemical Exposure

34 Module Interactive Avoid wearing gloves to open doors or touch door handles, for the safety of others. 1. What is the rationale for this rule? 2. By which route(s) could contaminated gloves lead to exposure? Module 4: Chemical Exposure

35 Chemical Toxicity “All substances are poisons; there is none which is not a poison. The right dose differentiates a poison from a remedy. ” -Paracelsus (1493 -1541) Acute Chronic Exposure Duration Sudden and severe exposure of high concentrations into the body Prolonged or repeated exposure over days, months or years. Concentrations vary; may be very little Health Risks Highly symptomatic, reversible health effects with medical treatment Typically non-symptomatic in early stages, irreversible health effects Examples Cyanide poisoning Concentrated vapors Lead poisoning Breathing dust Module 4: Chemical Exposure

36 Exposure Limits • OSHA Permissible Exposure Limit (PEL) – 8 -hour time weighted exposure for a worker’s lifetime – Based upon chemical toxicity • • Quantitative assessments are only safe means to determine exposure Smells and Odors are not good indicators of exposure Module 4: Chemical Exposure

37 Module Interactive Identify whether the condition is the result of acute or chronic exposure. 1. A burning sensation in the eyes from an ammonia splash 2. Numbness of the hands from decades of work with n-hexane 3. Tremors and coma from dermal absorption of an organophosphorous insecticide 4. Hepatitis from daily exposure, over a period of years, to chloroform Module 4: Chemical Exposure

38 Module Checkpoint • • • Know how to protect yourself from exposure to chemicals in the lab Understand routes of exposure Understand that chemicals can have acute and chronic health effects Module 4: Chemical Exposure

40 Hazard Communication Chemical Hygiene Plan Chemical Labeling Material Safety Data Sheets (MSDS) Module 5: Hazard Communication

41 The Lab Standard • OSHA Regulation 29 CFR 1910. 1450 “Occupational Exposure to Chemicals in the Laboratory” • Defines requirements for laboratories: – Chemical Hygiene Plan – On-the-job training for employees – Hazard data of chemicals used (Material Safety Data Sheets) Module 5: Hazard Communication

42 Chemical Hygiene Plan • Found on the EHS website – – • • • in Tab 6 of the Location Safety Manual http: //webster/EHS/safety. html Defines safe practices for chemical handling Identifies exposure control measures implemented Lists facility policies for treatment of exposure Module 5: Hazard Communication

43 Storage Cabinet and Room Labeling • Visible labeling on cabinets containing – Acids or corrosive materials – Flammable or combustible materials • Entrances to areas containing hazards should identify – Hazards present – Area supervisor and contact number Module 5: Hazard Communication

44 Hazard Warnings • Know the hazards before handling chemicals – – – – Harmful or Toxic Flammable or Combustible Corrosive Oxidizer Peroxidizable Reactive Particularly Hazardous Substances (carcinogens, teratogens, mutagens) Module 5: Hazard Communication Examples of EU pictoral warning symbols on chemical containers.

45 Labeling Hazardous Chemicals • Labeling is required on all containers – Manufacturer’s original container – Secondary/dispensing containers – Waste containers • Labeling on USDA secondary containers must include – Complete name(s) of chemicals and concentrations – Hazardous warning statement – Date opened (for peroxidizable chemicals) • Labels must be readable at all times; replace worn/degraded labels – Check solvent container labels Module 5: Hazard Communication

46 Chemical Labeling • Specific hazards are defined in the white portion of the diamond. Module 5: Hazard Communication

47 Hazard Communication Material Safety Data Sheets (MSDS) • Hazards and precautions • Physical and chemical incompatibilities • Emergency action procedures (First aid, accidental release) Module 5: Hazard Communication

48 Module 5: Hazard Communication

49 Module Interactive The MSDS in this presentation is in your informational pamphlet. Use the MSDS to answer the following questions. 1. Which section has first aid information? 2. List the fire extinguishers recommended for use. 3. What is the NFPA health hazard rating for acetonitrile? 4. Identify the exposure limit for this chemical. 5. What chemicals should not be stored with acetonitrile? Module 5: Hazard Communication

50 MSDS and Informational Sources • http: //webster/EHS/page 9. html • Each lab has an MSDS binder. Also the main library in the Conference room has a collection of MSDS sheets • Tox. Net – Additional source for chemical hazard data – http: //toxnet. nlm. nih. gov/ Module 5: Hazard Communication

51 Module Checkpoint • • • Recognize warnings on chemical containers and take necessary precautions Recognize warnings on doors and cabinets Refer to MSDS to learn important facts about the chemicals, especially hazards and exposure response procedures Module 5: Hazard Communication

53 Exposure Control Methods Engineering Controls Administrative Controls Personal Protective Equipment (PPE) Module 6: Exposure Control Methods

54 Exposure Controls • Control Hierarchy – Engineering Controls – Administrative Controls • Substitution – Personal Protective Equipment (PPE) Module 6: Exposure Control Methods

55 Chemical Fume Hood • Used for all work with volatiles • Optimal air flow conditions: – Keep baffles clear – Hood sash at recommended setting – Adjust baffles settings accordingly • Work at least 4 -6 inches into the hood • Not for chemical storage • Keep ignition sources out Module 6: Exposure Control Methods

56 Ventilation Controls • Local Exhaust Systems – Fume Hoods – Balance Enclosures – Snorkels • Dilution Ventilation – Low Toxicity Contaminants – Diffuse Contaminant Sources Module 6: Exposure Control Methods

57 Biosafety Cabinet Type II A • 70% air recirculated within enclosure (HEPA filtered) • 30% air HEPA filtered and exhausted into the room Type II B 2 • 100% air is HEPA filtered and exhausted to outside • For use with volatiles and radionuclides Module 6: Exposure Control Methods

58 Other Engineering Controls • Laminar flow, or “clean bench” • Ventilated balance enclosures • Ultraviolet (UV) lamps – UV light poses an exposure hazard – Use appropriate PPE Module 6: Exposure Control Methods

59 Personal Protective Equipment (PPE) • Laboratory Dress Code • Face and Eye Protection – – Safety Glasses Prescription Glasses Goggles Faceshield Module 6: Exposure Control Methods • Body Protection – – Lab Coat Apron Shoes Shoe covers • Hearing Protection – Earplugs – Earmuffs

60 Glove Selection • Use proper gloves for physical hazards: – Friction (work/sharps/broken glass) – Thermal (cryogens/heat) • Handling chemicals – – Latex Nitrile Vinyl Butyl • Based on chemical; Use compatibility chart Module 6: Exposure Control Methods

61 Assessment Activity Note the major hazards in your laboratory and specific control measures implemented in your lab. For example, with each chemical you will use, consider the following: 1. Is there a less hazardous chemical that could be used instead? 2. What engineering controls (e. g. , fume hood, glove box, etc. ) are needed when working with this chemical? 3. Are there any special procedures or SOPs that need to be followed when working with this chemical? 4. What PPE (e. g. , goggles, face shield, gloves (specify type), etc. ) must be worn when working with this chemical? Module 6: Exposure Control Methods

62 Module Checkpoint • • • Reduce your risk to exposure by knowing the hazards of chemicals and materials. Hazardous materials have both physical and health exposure risks. A proper combination of administrative and engineering controls helps minimize the risks of exposure. Select PPE based on the hazards of the substances you are using. Know basic emergency response measures. Module 6: Exposure Control Methods

63 Video Presentations

65 Chemical Management Example of the CIS/Pro Chemical Inventory Software Chemical Ordering and Inventory Chemical Storage Safe Handling Practices Module 7: Chemical Management

66 Chemical Ordering • Supervisors approve purchases – Supervisor approval for new materials • Received chemicals are processed – Container(s) barcoded – Entered into chemical inventory Module 7: Chemical Management

67 Chemical Storage • • • Use proper secondary containment Do not store chemicals on floor or in fume hoods Store chemicals below eye level Label stock solutions clearly Keep original labels on all containers Segregate chemicals by hazard never store alphabetically or randomly • Use shelving with seismic lips or restraints (for locations at risk to earthquakes) Module 7: Chemical Management

68 Chemical Storage Keep track of time-sensitive chemicals • Many solvents and reagents form unstable peroxides – Ethers, Furans • Dispose before they form peroxide crystals or dry out • Some chemicals must be stored in water – Picric acid (keep hydrated) • Date each time-sensitive chemical upon opening the container Module 7: Chemical Management

69 Chemical Storage When is it time to dispose of a chemical? • Chemicals without an identified use • Outdated chemicals • Unknowns* (missing or degraded label) • Unsafe or unstable chemicals* • Chemicals with unknown users and/or uses * Handle dried-up or crystalized chemicals with extreme care; peroxide crystals are highly unstable and can explode. Module 7: Chemical Management

70 Safe Handling Procedures • Wear proper personal protective equipment (PPE) • Review the material safety data sheets (MSDS) • Check container for special instructions • Know the location of emergency safety equipment • Minimize amounts of hazardous materials Module 7: Chemical Management

71 Safe Handling Procedures • Know the hazards associated with chemicals in the lab • Never smell or taste a chemical • Avoid direct contact with any chemical • Do not smoke, drink, eat, or apply cosmetics in the lab Module 7: Chemical Management

72 Safe Handling Procedures • Use adequate ventilation • Keep metal surfaces grounded • Do not remove labels on original containers • Label all dispensing containers – Chemical name – Primary hazards Module 7: Chemical Management

73 Module Interactive Use the Chemical Compatibility chart in your informational pamphlet to determine if the following chemicals are compatible for storage. 1. 2. 3. 4. 5. Organic solvent & organic pesticide/poison Oxidizer & organic acid Inorganic acid & water reactive Organic poison & inorganic poison Inorganic acid & oxidizer Module 7: Chemical Management

74 Module Checkpoint • • • Do your part to maintain the safety of chemicals in the laboratory Use the chemical inventory system to manage chemicals Use secondary containment for storage or transport to reduce the potential for a chemical spill or release Store chemicals only with compatible materials Dispose of obsolete chemicals Module 7: Chemical Management

76 Shipping and Transport Regulatory Agencies and Requirements Packaging and Labeling Module 8: Shipping and Transport

77 Regulatory Agencies • Department of Transportation (DOT) – • • DOT regulations apply to any shipment transported by vehicle on public roads (includes all city streets, highways and freeways) International Civil Aviation Organization (ICAO) International Air Transport Association (IATA) – IATA/ICAO are international agencies which are nearly harmonious with DOT and apply to air carriers Module 8: Shipping and Transport

78 Requirements for Shipping Chemicals • • • Classify and identify the material Package material appropriately Label, mark, and seal the package Complete a “Dangerous Goods Declaration” When in doubt, ask your Safety contact Module 8: Shipping and Transport

79 DOT Hazard Classes 1. 2. 3. 4. 5. 6. 7. 8. 9. Explosives Compressed Gases Flammable Liquids Flammable Solids Shipping Note Oxidizers Objects shipped with dry ice Toxic Substances must have a DOT Class 9 label on the outer packaging Radioactive Materials Corrosive Materials Miscellaneous Dangerous Goods Module 8: Shipping and Transport

80 Packaging, Labeling and Marking • Example: – – 0. 5 liters acetone Plastic inner packaging Absorbent Fiberboard box Acetone UN 1090 LTD QTY 3 Module 8: Shipping and Transport To: From:

81 Packaging, Labeling and Marking • • • Regulations Safety In case of spill or injury Liability for Damages Customs “Homeland Security” Module 8: Shipping and Transport

82 Module Checkpoint • • • Know how to properly prepare a package for shipping Use the appropriate warning and hazard labels Consult with the location Safety contact if you are unsure of the packaging requirements of your shipment Module 8: Shipping and Transport

84 Hazardous Waste Proper Disposal Storage and Labeling Waste Minimization Module 9: Hazardous Waste

85 Hazardous Waste • Chemical waste with these characteristics are considered “hazardous waste”: – Flammability – Corrosive materials – Reactive materials – Toxic substances – Specifically listed hazardous substances Module 9: Hazardous Waste

86 Proper Disposal • Waste must be properly disposed of • Never attempt to dispose waste by: – Pouring waste down the drain – Diluting with water to compromise criteria • East Bay sewer discharge limit of 5. 5 p. H • No strong acids down the drain – Evaporating volatiles in fume hoods – Disposing in non-regulated trash Module 9: Hazardous Waste

87 Disposing of Containers • A container may be thrown away or recycled if: – “Empty” by *California Law – Triple-rinsed – Did not contain an “extremely hazardous substance” (See Tab 4 of Safety Manual) – 5 gallons or less in capacity *California “empty” means nothing remains in the container Module 9: Hazardous Waste

88 Hazardous Waste Satellite Accumulation Areas (SAA) • Set the SAA location at or near the point of generation • Keep incompatible wastes segregated • Keep containers closed when not in use • Storage limit of 6 months • Submit request form to have removed or emptied Module 9: Hazardous Waste

89 HAZARDOUS WASTE GENERATOR INFORMATION- USDA, 800 Buchanan Street, Albany, CA 94710 Generator name_________ Federal Law Prohibits Improper Disposal If found, contact the nearest police, public safety authority or the US-EPA. Contents: Name Percent of total. HAZARD CLASSIFICATION Flammable, Corrosive, Toxic, Reactive Other Halogenated organic solvents are present WASTE FORM Solid, Liquid, Gas HANDLE WITH CARE Satellite Accumulation Area Date Started = ____ Date Sealed = ____ Module 9: Hazardous Waste Satellite Accumulation Areas • Select appropriate containers: – Closing glass or plastic bottles for strong acids and causitcs – Closing plastic or amber bottles for liquids/solvents – Sealable containers for dry waste • Requirements: – Proper labeling (example shown) – Secondary containment – Include the required information

90 1. Chemical waste generated in the labs is collected in a Satellite Accumulation Area (SAA). SAA 3. The waste is prepared for transport by a certified waste hauler to a Treatment/Storage/ Disposal Facility (TSDF). SAA WAA SAA 2. The waste is collected and transferred to a main Waste Accumulation Area (WAA). Module 9: Hazardous Waste

91 Hazardous Waste Pickup Information: • Pickups are scheduled by the EMS Committee • The waste containers must be prepared prior to pickup • Additional requirements apply to – – “Mixed” waste Lecture bottles Unstable or reactive chemicals Unknown waste • Remove containers from inventory before disposing • Non-compliant waste will not be picked up! Module 9: Hazardous Waste

92 Waste Minimization • Minimize chemical purchases – Use existing chemical stockroom supplies – Reduce orders to only what you need • Practice effective cleanup techniques • Participate in your facility’s recycling program Module 9: Hazardous Waste

93 Module Interactive From the list below, identify the necessary information required on the label of a Satellite Accumulation Area (SAA) waste container. • • • Generator name Color of waste Name(s) of contents Date started Weight of container Form of waste Module 9: Hazardous Waste • • • Size of container Presence of odors Date sealed NFPA diamond Location of container Date removed

94 Module Checkpoint • • Do your part to ensure that waste at the facility is properly managed and disposed Store your hazardous lab waste in a satellite accumulation areas (SAA) Use proper labeling on waste containers Reduce your facility’s waste output by ordering only what will be used Module 9: Hazardous Waste

96 Environmental Management System The EMS Mitigating Environmental Impacts Module 10: Environmental Management System

97 Environmental Management System (EMS) • • Goals to reduce environmental impacts Increases awareness Program monitored and audited Continuous improvement Module 10: Environmental Management System

98 Environmental “Fenceline” • Environmental Impacts – Air – Runoff & groundwater – Soil • Impacted Areas – – Residential Areas Schools Parklands Streams and Drainage Systems – Open Waters Module 10: Environmental Management System

99 Environmental Regulatory Oversight • Regulatory Oversight and Enforcement – EPA and State/EPA (DTSC) – Regional Water Quality Control Board (RWQCB) – Counties – Municipal Utilities Districts – Air Quality Management Districts Module 10: Environmental Management System

100 EMS Program Objectives Reduce: • Energy Consumption • Air Pollution • Atmospheric Impacts • Potential for Facility Contamination and Exposure • Risk and Mitigate Impacts of Environmental Release • Potential for Stormwater Contamination • Consumption of Natural Resources • Generation of Solid Wastes / Increase Recycling Module 10: Environmental Management System

101 Mitigating Environmental Impacts • Facility Measures – Abatement Control Devices – Energy-Efficient Lighting – Boiler and Generator Fuel Restrictions and Limits – Recycling and E-Waste • Laboratory Measures – Chemical Inventory System – Hazardous Waste Management – Sewer Discharge Limits and Monitoring Module 10: Environmental Management System

102 Mitigating Environmental Impacts • Pollution Prevention (P 2) – Reduction of pollution at the source – Minimizing/substituting hazardous materials – Efficient use of resources/raw materials • “Greening the Government” – Increased recycling and use of recycled materials – Reduce use of environmentally hazardous materials – Efficient use of power and fuels Module 10: Environmental Management System

103 Module Interactive Benzene is a flammable, volatile solvent. It is dangerous to the environment and is a known carcinogen. 1. Describe 3 ways this substance can find its way into the environment from a laboratory. 2. Identify a way (for each case) to help prevent this environmental impact from occurring. Module 10: Environmental Management System

104 Module 10: Environmental Management System

105 Module Checkpoint • • • Do your part to minimize the facility’s impact on the environment Be aware of your facility’s environmental programs Think about what waste materials you produce can be recycled Module 10: Environmental Management System

107 Emergency Response Emergency Situations Evacuation Procedures Responding to Injuries Module 11: Emergency Response

108 Emergency Support • • • First-Aid Response Team Spill Response Team O&M Service Desk Location Support Services Center Director’s Office After Hours Answering Service Outside Emergency Response Module 11: Emergency Response

109 Emergency Situations • What should you do in an emergency? You are expected to `SIN` at a minimum. • Safety - ensure your personal safety FIRST! • Isolation - alert co-workers to clear out and away from the emergency scene • Notification - call the appropriate emergency numbers Module 11: Emergency Response

110 Emergency Situations • • Building Alarm Earthquake Medical Emergency Civil Disturbance Power Outage Criminal Activity Industrial Accident Chemical, Biological or Radioactive Spills or Personal Contamination Module 11: Emergency Response

111 Know Your Surroundings Note the locations emergency safety equipment: • • • Spill Control Station First-Aid Kit Eyewash Station Emergency Shower Fire Extinguisher Fire Pull Station Module 11: Emergency Response

112 Using a Fire Extinguisher • • Intended only for small fires Notify co-workers of any fire situation Pull fire alarm (if appropriate) Use PASS Method – Pull pin, aim, squeeze, sweep • Notify your location safety contact Module 11: Emergency Response

113 Emergency Evacuations • Recognize the emergency announcement system and alarms • Familiarizing yourself with the evacuation maps • Knowing at least two escape routes • Elevators are not an option in an evacuation Module 11: Emergency Response

114 Emergency Evacuations In the event of an evacuation: • • • Proceed immediately to the assembly area Close corridor doors as you leave Check in with your supervisor Assist visitors and guests Do not leave the assembly area without permission Do not re-enter until the “All Clear” has been made Module 11: Emergency Response

115 Emergency Evacuations • In the event of an evacuation know the location of your assembly area. • Stay off the roadways to allow access for emergency vehicles. Module 11: Emergency Response

116 Module Interactive In the event of an emergency, such as a chemical spill, your immediate response is to SIN. Briefly, describe an action you would take in each step of SINning. Remember: Safety Isolation Notification Module 11: Emergency Response

117 Injuries & Lessons Learned Injury: An incubation flask became stuck to sticky strips on platform of incubator. A lab employee pried flask loose, causing it to shatter and lacerations to hand. Lesson Learned: If applying force to glassware, wear cutresistant gloves. Use minimal force at first to test the resistance of the sticky strip. Module 11: Emergency Response

118 Injuries & Lessons Learned Injury: An employee heated plant growth media in a threaded media bottle (cap loosened / Non Pyrex) then rapidly cooled the bottle under cold water causing bottle to explode resulting in deep incision over left eye. Lesson Learned: Wear eye protection; remove caps or plugs prior to heating media; quick cool glass only if it is tempered (Pyrex) Module 11: Emergency Response

119 Injuries & Lessons Learned Injury: An employee forced a glass pipette into pipetting device. The pipette shattered and the jagged end punctured the palm of hand, requiring several stitches. Lesson Learned: Seek a mechanical aid to load glass pipettes; if there is no mechanical aid, wear punctureresistant gloves for the task Module 11: Emergency Response

120 Injuries & Lessons Learned Injury: An employee received a blistered frostbite burn from contact with liquid nitrogen from a dewar tank Lesson Learned: Wear thermal gloves; Avoid contact with the valves and hoses, and stay clear of the dispensing end of the hose Module 11: Emergency Response

121 Module Checkpoint • • • Do your part to keep the building safe Report unusual occurrences to your supervisor Use the SIN system to respond to emergency situations Keep track of your guests at all times In an evacuation, exit the building immediately and go to your assembly area When an incident occurs, call for help right away Module 11: Emergency Response

123 Wrap Up • Report unsafe conditions, safety concerns, or safety suggestions Submit safety suggestions to supervisor, EH&S Website, or the Location EH&S Office Safety information is available from the EH&S website (http: //webster/EHS/safety. html) including: • • – – Safety Committee members and meeting minutes EH&S Contacts Safety policies and chemical hazard information And much more Module 11: Emergency Response

124 Challenge Exam • • Print and complete the attached exam: NEO Lab Quiz Submit your completed exam sheet to either your: – – • Location Safety Office Collateral Duty Safety Officer (CDSO) Thank you for taking this training. Challenge Exam