1 KETA series Imaging System Chemiluminescence C Gel

1 KETA series Imaging System Chemiluminescence (C) Gel Documentation (G) Multi-Purpose (M) 2021/10/21

2 Features of KETA series systems - Advanced, Automatic controlling system • Robotic controlling system including: Motorized lens and filter wheel, auto sample stage(option) • MC 100/U 10 LED: Multi color Epi-LED provides the basic fluorescence experiment. U 10 white LED for sample placement. 2021/10/21

3 • KD 100 dark room: Absolute light-tight dark room suitable for chemiluminescence experiment. AS 100 Auto stage Leakage proof folding Smart Latch Optional SELM 200 Upgradeable high intensity Xenon light as excitation light of live plant and animal fluorescence detection. Safety Switch Safety inter lock 2021/10/21

4 • Status display Signal light • Customize idling time setting • MD 25 K 312 nm UV Transilluminator (Also in KETA G) Intensity of UV light was controlled by computer • Dedicate controlling software: Magic 1 D & Magic Chemi 2021/10/21

5 KETA M Robotic Models: M: 2/3”, Peltier cooled ML: 2/3”, 2 stage Peltier cooled MLX: 2/3”, 16 bits, Peltier cooled Outstanding performance on gel documentation and highly sensitive on Chemi experiments. 2021/10/21

6 KETA M Gel Analysis Fluorescence Chemiluminescence 2021/10/21 KETA MLX +++++ +++++ ++++

7 Camera of KETA M Series K 12 CHS K 16 CH CCD size 2/3" Depth 12/16. A/D bit 12 /16. A/D bit 16 bit Grayscale 4096 /65536 65535 Resolution 1360 X 1024 2048 X 2048 S/N Cooling >62 d. B 6. 45 μm x 6. 45 μm Peltier >67 d. B 6. 45 μm x 6. 45 μm 2 -stage Peltier >69 d. B 7. 4 μm x 7. 4 μm Peltier ↓Ambient -30℃ -45℃ -40℃ Megapixels 1. 4 4. 0 Communication USB 2. 0 Pixel Size 2021/10/21

8 QE value K 12 CH/K 12 CHS K 16 C/K 16 CH Dark Current : K 12 CH/K 12 CHS/K 16 CH Lower than 0. 25 e/pixel/s@ 20℃ Ambient 2021/10/21

9 KETA M Configuration Camera KETA MLX K 12 CHS K 16 CH Motorized Filter Wheel 5 5 5 MC 100 LED (W/R/G/B) Standard MC 100 -1 (W/G/B/UV 365 nm) Option AS 100 Option Standard Magic 1 D & Magic Chemi Standard Communication USB 2. 0 Thermal printer Option SELM Module 200 Option Lens f 1. 4, 11. 5 -69 mm 2021/10/21 f 1. 4, 11. 5 -69 mm

10 Typical Application for KETA M l Gel documentation l Colony counting l Microtiter plate imaging l Spot density calculation l Photographic imaging l 1 D analysis l Fluorescence detection l Chemiluminescence detection l Optional SELM 200 for live imaging 2021/10/21

11 Sample Detection Protein • Coomassie Blue • Silver Stain • SYPRO Orange • SYPRO Ruby • SYPRO Red • Fluorescein • Q-Dot 2021/10/21 Nucleic Acid • Ethidium Bromide • SYPRO Green • SYPRO Gold • Texas Red • Gel Star Chemiluminescent • ECL, ECL Plus • ECL Advance, • ECL Plex • CDP star, • Q-dot • Super signal • CSPD

12 Applications KETA ML SYBR Green I stain: Excited with: 312 nm Trans. UV, Filter: WK 102 SYPRO Red Excited stain: with: 312 nm Trans. UV, Filter: WK 105 2021/10/21

13 Chemiluminescence Experiment (A) Sample: β-actin in MCF 7 cell lysate Image model: ML Stain: plus-ECL (Perkin Elmer) Exposure time: 20 seconds Binning: Off 2021/10/21 (B) Sample: GST pure protein Image model: ML Stain: ECL Enhanced (Millipore) Exposure time: 20 seconds Binning: Off

14 • KETA M system: High performance gel doc and good sensitivity of Chemi detection. • KETA ML system: 2 -stage Peltier cooled system provides best cooling effect for the camera. ↓ noise, ↑performance. ÞMost profitable systems for sale. • KETA MLX system: Truly 16 bits CCD with 4. 2 Megapixels provides the best observation on both Chemi- and Fluordetection. 2021/10/21

15 Specs to defeat competitors l AS 100 sample stage 1. Easy for adjust the height of sample 2. Clear in focusing 3. Change samples fast and easy 4. Prevent cross contamination 5. Easy to enlarge the observation area l 2 nd stage Peltier camera 1. Cheapest among the same level image systems 2. Absolute low temperature 3. Low noise 4. High S/N ratio 5. Good Chemi detection ability l Upgradeable to live imaging system 2021/10/21

16 Magic 1 D Controls Dolphin series and KETA series, respectively 2021/10/21

17 Features of 1 D software • Easy image capturing • Multiple file format supported: JPG, BMP, JPEG, DFN, TIFF, PCX, GIF, TGA image • Automatic data analysis (Within 1 minute) • Traceable image enhancement function • Intelligent colony counting • Comprehensive microtiter plate analysis • Accurate spot density calculation • 3 -D display image profile • Support GLP/GMP • Image blending function 2021/10/21

18 Magic Chemi Software Enhancing on Image Capturing Tools • Image capture control panel • Batch capture for different exposure time • Dyna. View capture function for accumulate multi-exposure signal • Force cooling design ensure the best S/N ratio • Darkroom calibration eliminate all undesired noise from the cabinet • Binning and Gain function for hard-detectible signal 2021/10/21

19 Image Capturing l Dyna. View: l Batch Capture: Exposure Time pic 1 pic 2 pic 3 Time Allow users to select the best-desired image with optimal exposure time from the captured set. 2021/10/21 + + + Time Allow users to accumulate the multi capture signal with same exposure time.

20 • Gel image analysis: DNA agarose gel, sequencing gel, blotting membrane, Protein SDS-PAGE gels, X-Ray film and photo etc. – Lane profile with lane image alignments – Ability to identify smiling gel accurately – Specialized X-axis band analysis function – Precise sample info report 2021/10/21

21 • Colony counting: -Auto colony counting -intelligent colony distinguish • Microtiter plate assay: Includes 48, 96, 384, 1536 wells micro-plate assay and various dot-blot and slot-blot formats. 2021/10/21

22 • Spot image analysis: Suitable for irregular shape sample analysis, including 2 -D gel sample and other special purpose assays. 2021/10/21

23 Thank you!! 2021/10/21