1 Hsp heat shock protein 90 Hahn JS

1. Hsp (heat shock protein) 90 작용 기작 Hahn JS (2009) The Hsp 90 chaperone machinery: from structure to drug development. BMB Rep 42(10): 623 -630

2. 암세포에서 Hsp 90의 발현 확인 면역 조직 화학 염색법 일반 조직과 유방암 조직을 대상 으로 단일클론항체인 anti. Hsp 70 과 anti-Hsp 90 를 이용하여 면역조직 화학 염색법을 시 행 Hee Joon Kang, Mi-Kyoung Hong, Sung Ku Jung, Lee Su Kim The Role of Heat Shock Proteins 70/90 as Potential Molecular Therapeutic Targets in Breast Cancer J Breast Cancer 2007; December 10 (4): 231 -40

2. 암세포에서 Hsp 90의 발현 확인 Western blot analysis 1 : MDA-MB 435 2 : MDA-MB 231 3 : MDA-MB 231 LC 3 4 : MDA-MB 231 GFP 5 : MDA-MB 231 BR 3 6 : MCF-7 7 : T-47 D Hee Joon Kang, Mi-Kyoung Hong, Sung Ku Jung, Lee Su Kim The Role of Heat Shock Proteins 70/90 as Potential Molecular Therapeutic Targets in Breast Cancer J Breast Cancer 2007; December 10 (4): 231 -40 호르몬 수용체 음성인 세포주 MDA-MB 435(1), MDA-MB 231 (2) 호르몬 수용체 양성인 세포주 MCF-7(6), T-47 D (7) MDA-MB 231(2) 을 GFP 로 형질 전환한 세포주 MDA-MB 231 GFP (4) 뇌와 폐로 장기 특이적인 전이를 일으킨 세포주 MDA-MB 231 BR 3(5), MDA-MB 231 LC 3 (3)

3. hsp 90 억제제 직접적 효과 Luke Whitesell & Susan L. Lindquist Hsp 90 and the Chaperoning of Cancer: Inhibiting Hsp 90 Function Nat Rev Cancer. 2005; 5(10): 761 -772 간접적 효과 heat shock factor (HSF) 의 활성화-> HSP 70 발현 -> 세포 사멸 억제, 스트레스로부터 세포를 보호

3. hsp 90 억제제 MTT assay 확인 Hee Joon Kang, Mi-Kyoung Hong, Sung Ku Jung, Lee Su Kim The Role of Heat Shock Proteins 70/90 as Potential Molecular Therapeutic Targets in Breast Cancer J Breast Cancer 2007; December 10 (4): 231 -40

3. hsp 90 억제제 Hsp 90 억제제의 효과 증진 방법 Confocal Microscope를 이용하여 관찰한 ALF와 Cytochrome C Jun-Young Park, D. D. S. , M. S. D. , Se-Jin Park, D. D. S. , M. S. D. , In-Ryoung Kim, M. S. , Ph. D. , Apoptotic Effect of Co-Treatment with Valproic Acid and 17 AAG on Human Osteosarcoma Cells 대한구강내과학회지Vol. 36, No. 1, 2011

6. 참고문헌 Ⅳ. 참 고 문 헌 [1] Luke Whitesell & Susan L. Lindquist Hsp 90 and the Chaperoning of Cancer: Inhibiting Hsp 90 Function Nat Rev Cancer. 2005; 5(10): 761 -772 [2] Ellen A. A. Nollen and Richard I. Morimoto Chaperoning signaling pathways: molecular chaperones as stress-sensing ‘eat shock’proteins Journal of Cell Science 115, 2809 -2816 (2002) [3] Hahn JS (2009) The Hsp 90 chaperone machinery: from structure to drug development. BMB Rep 42(10): 623 -630 [4] Avrom J. Caplan What is a co-chaperone? Cell Stress Chaperones. 2003 April; 8(2): 105– 107. [5] D Mahalingam 1, R Swords 1, J S Carew 1, S T Nawrocki 1, K Bhalla 2 and F J Giles 1 Targeting Hsp 90 for cancer therapy British Journal of Cancer (2009) 100, 1523– 1529 [6] Hee Joon Kang, Mi-Kyoung Hong, Sung Ku Jung, Lee Su Kim The Role of Heat Shock Proteins 70/90 as Potential Molecular Therapeutic Targets in Breast Cancer J Breast Cancer 2007; December 10 (4): 231 -40 [7] Jun-Young Park, D. D. S. , M. S. D. , Se-Jin Park, D. D. S. , M. S. D. , In-Ryoung Kim, M. S. , Ph. D. , Apoptotic Effect of Co-Treatment with Valproic Acid and 17 AAG on Human Osteosarcoma Cells 대한구강내과학회지Vol. 36, No. 1, 2011 [8] Masahiro Waza 1, 2, Hiroaki Adachi 1, 2, Masahisa Katsuno 1, Makoto Minamiyama 1, Chen Sang 1, Fumiaki Tanaka 1, Akira Inukai 1, Manabu Doyu 1 & Gen Sobue 17 -AAG, an Hsp 90 inhibitor, ameliorates polyglutamine-mediated motor neuron degeneration Nature Medicine 11, 1088 1095 (2005) [9] Wenjie Luo, Fei Dou , Anna Rodina Sophorn Chip, Joungnam Kim, Qi Zhao, Kamalika Moulick , Julia Aguirre , Nian Wu , Paul Greengard , and Gabriela Chiosis Roles of heat-shock protein 90 in maintaining and facilitating the neurodegenerative phenotype in tauopathies PNAS May 29, 2007 vol. 104 no. 22 9511 -9516 [10] Ruth A. Bodner, Tiago Fleming Outeiro, Stephen Altmann, Michele M. Maxwell, Stephanie H. Cho, Bradley T. Hyman , Pamela J. Mc. Lean , Anne B. Young, David E. Housman , and Aleksey G. Kazantsev Pharmacological promotion of inclusion formation: A therapeutic approach for Huntington’s and Parkinson’s diseases PNAS March 14, 2006 vol. 103 no. 11 4246 -4251

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