1 Diffusion Rate CHEMICAL DIFFUSION IN AGAR IF

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1) Diffusion Rate CHEMICAL DIFFUSION IN AGAR IF YOU CAN SEE THIS MESSAGE YOU

1) Diffusion Rate CHEMICAL DIFFUSION IN AGAR IF YOU CAN SEE THIS MESSAGE YOU ARE NOT IN “SLIDE SHOW” MODE. PERFOMING THE LAB IN THIS MODE WILL NOT ALLOW FOR THE ANIMATIONS AND INTERACTIVITY OF THE EXERCISE TO WORK PROPERLY. TO CHANGE TO “SLIDE SHOW” MODE YOU CAN CLICK ON “VIEW” AT THE TOP OF THE PAGE AND SELECT “SLIDE SHOW” FROM THE PULL DOWN MENU. YOU CAN ALSO JUST HIT THE “F 5” Click here to print a Data KEY. Click Here To Start Experiment #1 Table you will need for this experiment

Diffusion is a process of equalization which involves movement of molecules from an area

Diffusion is a process of equalization which involves movement of molecules from an area of high concentration to an area of low concentration Click Mouse Here to View Next Text on the Blackboard

This exercise investigates diffusion as it applies to movement of particles in a semi-solid

This exercise investigates diffusion as it applies to movement of particles in a semi-solid material called agar Click Mouse Here to View Next Text on the Blackboard

Molecules are in a constant state of motion The motion of these molecules are

Molecules are in a constant state of motion The motion of these molecules are influenced by many factors Click Mouse Here to View Next Text on the Blackboard

FACTORS INFLUENCING DIFFUSION 1) Kinetic Energy 2) Nature of the environment 3) Size of

FACTORS INFLUENCING DIFFUSION 1) Kinetic Energy 2) Nature of the environment 3) Size of the molecules Click Mouse Here to View Next Text on the Blackboard

KINETIC ENERGY The driving force which causes the molecules to move Click Mouse Here

KINETIC ENERGY The driving force which causes the molecules to move Click Mouse Here to View Next Text on the Blackboard

NATURE OF THE ENVIRONMENT The agar environment we will be using is a semisolid

NATURE OF THE ENVIRONMENT The agar environment we will be using is a semisolid material through which we will measure the rate of diffusion of substances with different molecular size Click Mouse Here to Perform the Exercise on Chemical Diffusion in Agar

SIZE OF THE MOLECULES Smaller molecules move faster than larger molecules Click Mouse Here

SIZE OF THE MOLECULES Smaller molecules move faster than larger molecules Click Mouse Here to View Next Text on the Blackboard

LAB REPORT You will need to supply the following in your lab report for

LAB REPORT You will need to supply the following in your lab report for this experiment #1 1) Data Table for Diffusion of Different Molecular Weights 2) Answers to questions 1 -4 Click Mouse Here to Perform the Exercise on Chemical Diffusion in Agar Click here to print a Data Table you will need for this experiment

Procedure Test tube rack Click Mouse Here to View Next Instructions on the Blackboard

Procedure Test tube rack Click Mouse Here to View Next Instructions on the Blackboard 1) Obtain two test tubes with agar *You can obtain the test tubes by clicking on the test tube rack on the shelf to the left

Procedure 2) Mark the test tubes as #1 and #2 *If you click on

Procedure 2) Mark the test tubes as #1 and #2 *If you click on the pencil lying on the shelf to the left you can mark the tubes 1 Click Mouse Here to View Next Instructions on the Blackboard 2

Procedure 3) Add a small amount of methylene blue to test tube #1 *You

Procedure 3) Add a small amount of methylene blue to test tube #1 *You can click on the blue eyedropper lying on the shelf to the left to add the dye 1 Click Mouse Here to View Next Instructions on the Blackboard 2

Procedure 4) Quickly click on the START button on the timer and record on

Procedure 4) Quickly click on the START button on the timer and record on your printed Data Sheet every 15 minutes the distance the dye has moved through the agar *You can measure the progress of the dye by observing the mm marks on the test tubes 1 START 00: 00 60: 00 55: 00 50: 00 45: 00 40: 00 35: 00 30: 00 25: 00 20: 00 15: 00 10: 00 05: 00 Click Mouse Here to View Next Instructions on the Blackboard 2 Click here if you messed up and would like to try this experiment again

Procedure 5) Add a small amount of potassium permanganate to test tube #2 *You

Procedure 5) Add a small amount of potassium permanganate to test tube #2 *You can click on the green eyedropper lying on the shelf to the left to add the dye 1 Click Mouse Here to View Next Instructions on the Blackboard 2

Procedure 6) Quickly click on the START button on the timer placed on the

Procedure 6) Quickly click on the START button on the timer placed on the table and record every 15 minutes the distance the dye has moved through the agar *You can measure the progress of the dye by observing the mm marks on the test tubes 1 START 00: 00 60: 00 55: 00 50: 00 45: 00 40: 00 35: 00 30: 00 25: 00 20: 00 15: 00 10: 00 05: 00 Click Mouse Here to View Next Instructions on the Blackboard 2 Click here if you messed up and would like to try this experiment again

DIFFUSION OF DYE IN AGAR Click the Play button to view the diffusion of

DIFFUSION OF DYE IN AGAR Click the Play button to view the diffusion of dye in an agar plate. Which chemical would you propose is smaller the RED or BLUE? Click Here to View the Next Slide

DATA At this point you should have collected diffusion distance data for methylene blue

DATA At this point you should have collected diffusion distance data for methylene blue and potassium permanganate Include your data tables with your lab report when you are finished Data Table for Diffusion of Different Molecular Weights Methylene blue (molecular weight of 320) Time 15 min 30 min 45 min 60 min Distance Potassium permanganate (molecular weight of 158) Time 15 min 30 min Distance Click Mouse Here to View Next Instructions on the Blackboard 45 min 60 min Click Here to view a page to print for your Data Table

Questions Answer the questions (1 -4) for this experiment Hand your answers in with

Questions Answer the questions (1 -4) for this experiment Hand your answers in with your lab report 1) What are three things which influence the movement of molecules (and particles)? 2) In agar, what size of molecules move the fastest? 3) Which dye, the methylene blue or the potassium permanganate, diffused at the fastest rate? 4) Why did one dye diffuse faster than the other? Click Mouse Here to View Next Instructions on the Blackboard

At this point you have finished experiment #1 for this lab Would you like

At this point you have finished experiment #1 for this lab Would you like to end your session or continue to the next experiment? End this Session Continue to Experiment #2 (PASSIVE MOVEMENT) Continue to Experiment #3 (OSMOSIS AND DIALYSIS)

Data Table for Diffusion of Different Molecular Weights Methylene blue (molecular weight of 320)

Data Table for Diffusion of Different Molecular Weights Methylene blue (molecular weight of 320) Time 15 min 30 min 45 min 60 min Distance in mm Potassium permanganate (molecular weight of 158) Time 15 min 30 min 45 min 60 min Distance in mm Click Here to Return to Experiment #1 You may also use this hyperlink to Print this Data Table if you are Online