1 Computer Filename Protsynb ppt Protein Synthesis Material
1 Computer Filename: Protsynb. ppt Protein Synthesis Material for Introductory Microbiology 9/30/2020
2 Overview þGenes are composed of DNA within the chromosome þGenes code for proteins þDNA is transcripted into RNA þRNA is translated into protein by ribosomes þGenes regulated by control of RNA production 9/30/2020
3 Lecture Outline Protein synthesis þt. RNA structure adding amino acids to t. RNA þribosome structure þribosome attachment þprotein synthesis : initiation, elongation, attachment þ 9/30/2020
4 Concepts from OAC þStructure & Function of DNA & RNA þGenetic code þTranscription & translation þProtein synthesis 9/30/2020
5 Background Terms & Concepts *Structure of DNA & RNA þRibosomes þEnzymes 9/30/2020
6 CODONS þ sequences of three adjacent nucleotides that code for one amino acid or polypetide termination þ 64 codons encrypt of ~ 20 amino acids þ degenerate ACU, ACC, ACA, ACG threonine þ Start codon AUG (also codes for methionine) þ Stop codon UAA, UAG, UGA (nonsense codons) 9/30/2020
7 Interpreting the Genetic Code þ Two step process : Transcription - translation þ Transcription DNA --> RNA þ Translation RNA--> protein 9/30/2020
8 Transcription Production of an RNA copy of the gene þ occurs in nucleus þ DNA unravels- single strand þ RNA Polymerase binds to Promotor site þ Reads to stop codon þ assembles complementary single strand of RNA (primary RNA) þ Disengages when reaches stop codon 9/30/2020
9 Protein Synthesis þFormation of an initiation complex þChain elongation þChain termination 9/30/2020
10 9/30/2020 Concept Map: Protein Synthesis
11 9/30/2020 Initiation of Protein Synthesis
12 9/30/2020 Peptide Elongation
13 9/30/2020 t. RNA-AA Binding
14 9/30/2020 Protein Membrane Signal Sequences
15 9/30/2020 Cytoplasmic signal sequences
16 9/30/2020 Transmembrane Sequences
17 9/30/2020 EM of ER
18 9/30/2020 Golgi Signalling
19 Overview of Protein Synthesis 9/30/2020
20 9/30/2020 Transcription-translation Overview
- Slides: 20
