1 Agarose Gel DNA Electrophoresis Running time 52
- Slides: 7
1% Agarose Gel DNA Electrophoresis Running time : 52 minutes 1 Kb 100 Kb Scale in Cm Distance measured from the well to the top of the second band: 4, 5 cm Running voltage: 100 Volt Solidification time: 14 minutes Composition: 250 mikro. L buffer TAE + 2, 5 gram Agarose + 20 mikro. L Ethidium Bromide Second Band
2% Agarose Gel DNA Electrophoresis Running time : 52 minutes Distance measured from the well to the top of the second band: 3. 4 cm 1 Kb 100 Kb Scale in Cm Total Running time: 62 minutes Total distance measured: 4. 5 cm Running voltage: 100 Volt Solidification time: 21 minutes Composition: 500 m. L buffer TAE + 10 gram Agarose + 40 mikro. L Ethidium Bromide Second Band
3% Agarose Gel DNA Electrophoresis Running time : 52 minutes Distance measured from the well to the top of the second band: 2. 9 cm 1 Kb 100 Kb Scale in Cm Total Running time: 68 minutes Total distance measured: 4. 5 cm Running voltage: 100 Volt Solidification time: 17 minutes Composition: 250 mikro. L buffer TAE + 7. 5 gram Agarose + 40 mikro. L Ethidium Bromide Second Band
Overall comparison 1% Cross sections of 3 different concentration of DNA Agarose Gel are illuminated under UV light 2% 3%
Overall Comparison Increasing the concentration of Agarose gel decreases the electrpphoretic mobility of DNA 1% 2% 3% Larger Fragments are better resolved in the low agarose concentration while higher concentration allow resolution of smaller DNAs 1 kb 100 bp
Comparison 5 mikro. L of 1 Kb DNA Ladder in 1%, 2% and 3% Agarose Gel Electrophoresis 1% 2% 3% Wells level 3, 0 kb 2, 0 kb
Comparison 5 mikro. L of 100 bp DNA Ladder in 1%, 2% and 3% Agarose Gel Electrophoresis 1% 2% 3% Wells level 1000 bp 500 bp
