1 3 D Structure of Immunoglobulins Hugh B
1 3 D Structure of Immunoglobulins Hugh B. Fackrell Filename: Kuby 05 b 7. ppt 5/22/2021
2 ä Assigned Reading ä Content Outline ä Performance Objectives ä Key terms ä Key Concepts ä Short Answer Questions 5/22/2021
3 ASSIGNED READING ä Chapter pp 5/22/2021
4 Antibody structure ä Special antibodies to study structure ä Myeloma proteins ä Bence Jones Proteins ä Monoclonal Antibodies ä 3 D structure of antibodies ä Binding site ä Variability in structure 5/22/2021
5 Angels & Devils 5/22/2021
6 EM of Ig. A 5/22/2021
7 Antibody flexibility 5/22/2021
8 Antibody: Space filling model 5/22/2021
9 Mulptiple Myeloma ä Disease ä Plasmocytoma ä Cancer of antibody producing cell ä Symptoms ä recurrent infections, anaemia, fractures, pain, bone lesions ä Detection ä Electrophoresis, X-rays, blood smear 5/22/2021
10 Ig. G Crystal 5/22/2021
11 Myeloma Induction 5/22/2021
12 Bence Jones Protein ä Homogenous light chains ä In urine of myeloma patients ä Easily purified ä Insoluble at 60 -70 o. C ä Soluble at 80 o. C 5/22/2021
13 Monoclonal Antibodies ä Add information here 5/22/2021
14 Antibody model 5/22/2021
15 Problems with flexibility ä Difficult to determine entire structure ä Hinge flexibility ä Fragment ä Study Fragment ä Assemble composite 5/22/2021
16 Hinge Prolines 5/22/2021
17 Ig. G: Enzyme hydrolysis 5/22/2021
18 Bence Jones Light chains ä Simplest fragments to isolate ä Easily crystallized ä Mimics Fab 5/22/2021
19 Bence Jones Monomer 5/22/2021
20 Bence Jones Dimer 5/22/2021
21 Immunoglobulin fold ä Signature structure common to all immunoglobulins ä 2 beta sheets ä held by disulfide bond ä Variations in detailed structure set specificity 5/22/2021
22 Ig Fold: Variable domains 5/22/2021
23 Ig fold: Constant domains 5/22/2021
24 Antigen Binding Site 5/22/2021
25 Structure of Ig. G 5/22/2021
26 Ig Fold: hypervariable regions 5/22/2021
27 Vitamin K bound to Ig. G 5/22/2021
28 Antigen binding to Antibody 5/22/2021
29 Antibody Strucutre: Internal motion 5/22/2021
30 Antibody Structure: Internal movement 5/22/2021
31 Antigen in Antibody 5/22/2021
32 Hypervariable Regions ä Discovery ä KW plots ä Light chains ä Heavy chains ä CDR vs HVR 5/22/2021
33 Kabat & Wu Plots ä Why ä demonstrate details of variability of protein sequence of similar proteins ä Results ä Variability = 1. 0 >> no variation ä Variability = 400 >> 20 different AA Variability = Number different amino acids Frequency of most common AA 5/22/2021
34 HV: light chains 5/22/2021
35 KW plot heavy chains 5/22/2021
36 HV: Ig. G 5/22/2021
37 KW Heavy chains CDR 5/22/2021
38 KW Light chains CDR 5/22/2021
39 Ig Superfamily 5/22/2021
40 Isotypes Allotypes Idiotypes 5/22/2021
41 Allosera Production 5/22/2021
42 Isotypes, Allotype Idiotype 5/22/2021
43 The End 5/22/2021
44 Performance Objectives Key terms, concepts short answers 5/22/2021
45 SHORT ANSWER QUESTIONS 5/22/2021
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